BMRB Entry 17179

Name: NMR Studies of the Backbone Flexibility and Structure of Human Growth Hormone: A Comparison of High and Low pH Conformations
Published: 2010-11-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17179

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Studies of the Backbone Flexibility and Structure of Human Growth Hormone: A Comparison of High and Low pH Conformations

Deposition date:

2010-09-10

Original release date:

2010-11-10

Authors:

Kasimova, Marina; Kristensen, Soren; Howe, Peter; Christensen, Thorkild; Matthiesen, Finn; Petersen, Jorgen; Sorensen, Hans; Led, Jens

Citation:

Citation: Kasimova, Marina; Kristensen, Soren; Howe, Peter; Christensen, Thorkild; Matthiesen, Finn; Petersen, Jorgen; Sorensen, Hans; Led, Jens. "NMR Studies of the Backbone Flexibility and Structure of Human Growh Hormone: A Comparison of High and Low pH Conformations" J. Mol. Biol. 318, 679-695 (2002).
PubMed: 12054815

Assembly members:

Assembly members:
rhGH, polymer, 191 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rhGH: FPTIPLSRLFDNAMLRAHRL HQLAFDTYEEFEEAYIPKEQ KYSFLQNPQASLCFSESIPT PSNREQAQQKSNLQLLRISL LLIQSWLEPVGFLRSVFANS LVYGASDSDVYDLLKDLEEG IQTLMGRLEDGSPRTGQIFK QTYSKFDANSHNDDALLKNY GLLYCFRKDMDKVETFLRIV QCRSVEGSCGF

Data sets:

chemical_rates
- Chemical_rate_list_1
- Chemical_rate_list_2

Data type	Count
kinetic rates	182

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rhGH	1

Entities:

Entity 1, rhGH 191 residues - Formula weight is not available

1

PHE

PRO

THR

ILE

PRO

LEU

SER

ARG

LEU

PHE

2

ASP

ASN

ALA

MET

LEU

ARG

ALA

HIS

ARG

LEU

3

HIS

GLN

LEU

ALA

PHE

ASP

THR

TYR

GLU

4

PHE

GLU

ALA

TYR

ILE

PRO

LYS

GLU

GLN

5

LYS

TYR

SER

PHE

LEU

GLN

ASN

PRO

GLN

ALA

6

SER

LEU

CYS

PHE

SER

GLU

SER

ILE

PRO

THR

7

PRO

SER

ASN

ARG

GLU

GLN

ALA

GLN

LYS

8

SER

ASN

LEU

GLN

LEU

ARG

ILE

SER

LEU

9

LEU

ILE

GLN

SER

TRP

LEU

GLU

PRO

VAL

10

GLY

PHE

LEU

ARG

SER

VAL

PHE

ALA

ASN

SER

11

LEU

VAL

TYR

GLY

ALA

SER

ASP

SER

ASP

VAL

12

TYR

ASP

LEU

LYS

ASP

LEU

GLU

GLY

13

ILE

GLN

THR

LEU

MET

GLY

ARG

LEU

GLU

ASP

14

GLY

SER

PRO

ARG

THR

GLY

GLN

ILE

PHE

LYS

15

GLN

THR

TYR

SER

LYS

PHE

ASP

ALA

ASN

SER

16

HIS

ASN

ASP

ALA

LEU

LYS

ASN

TYR

17

GLY

LEU

TYR

CYS

PHE

ARG

LYS

ASP

MET

18

ASP

LYS

VAL

GLU

THR

PHE

LEU

ARG

ILE

VAL

19

GLN

CYS

ARG

SER

VAL

GLU

GLY

SER

CYS

GLY

20

PHE

Samples:

sample_1: rhGH0.7 – 1 mM; H2O 99.996%

sample_conditions_1: pH: 7.0; pressure: ambient atm; temperature: 305 K

sample_conditions_2: pH: 2.7; pressure: ambient atm; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions
HN exchange	sample_1	isotropic	sample_conditions_2
HN exchange	sample_1	isotropic	sample_conditions_1

Software:

software, na - na

NMR spectrometers:

Varian Unity Inova 500 MHz