BMRB Entry 17162

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17162

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for a photochromic fluorescent protein Dronpa in the bright state
Deposition date:
2010-09-01
Original release date:
2010-11-09
Authors:
Mizuno, Hideaki; Mal, Tapas; Waelchli, Markus; Fukano, Takashi; Ikura, Mitsuhiko; Miyawaki, Atsushi
Citation:

Citation: Mizuno, Hideaki; Mal, Tapas Kumar; Walchli, Markus; Fukano, Takashi; Ikura, Mitsuhiko; Miyawaki, Atsushi. "Molecular basis of photochromism of a fluorescent protein revealed by direct (13)C detection under laser illumination."  J. Biomol. NMR 48, 237-246 (2010).
PubMed: 21052778

Assembly members:

Assembly members:
Dronpa, polymer, 225 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: stony corals   Taxonomy ID: 301887   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Echinophyllia not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dronpa: GSHMSVIKPDMKIKLRMEGA VNGHPFAIEGVGLGKPFEGK QSMDLKVKEGGPLPFAYDIL TTVFXNRVFAKYPENIVDYF KQSFPEGYSWERSMNYEDGG ICNATNDITLDGDCYIYEIR FDGVNFPANGPVMQKRTVKW EPSTEKLYVRDGVLKGDVNM ALSLEGGGHYRCDFKTTYKA KKVVQLPDYHFVDHHIEIKS HDKDYSNVNLHEHAEAHSEL PRQAK

Data sets:
Data typeCount
13C chemical shifts392
15N chemical shifts196
1H chemical shifts196

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Dronpa1

Entities:

Entity 1, Dronpa 225 residues - Formula weight is not available

1   GLYSERHISMETSERVALILELYSPROASP
2   METLYSILELYSLEUARGMETGLUGLYALA
3   VALASNGLYHISPROPHEALAILEGLUGLY
4   VALGLYLEUGLYLYSPROPHEGLUGLYLYS
5   GLNSERMETASPLEULYSVALLYSGLUGLY
6   GLYPROLEUPROPHEALATYRASPILELEU
7   THRTHRVALPHEGYCASNARGVALPHEALA
8   LYSTYRPROGLUASNILEVALASPTYRPHE
9   LYSGLNSERPHEPROGLUGLYTYRSERTRP
10   GLUARGSERMETASNTYRGLUASPGLYGLY
11   ILECYSASNALATHRASNASPILETHRLEU
12   ASPGLYASPCYSTYRILETYRGLUILEARG
13   PHEASPGLYVALASNPHEPROALAASNGLY
14   PROVALMETGLNLYSARGTHRVALLYSTRP
15   GLUPROSERTHRGLULYSLEUTYRVALARG
16   ASPGLYVALLEULYSGLYASPVALASNMET
17   ALALEUSERLEUGLUGLYGLYGLYHISTYR
18   ARGCYSASPPHELYSTHRTHRTYRLYSALA
19   LYSLYSVALVALGLNLEUPROASPTYRHIS
20   PHEVALASPHISHISILEGLUILELYSSER
21   HISASPLYSASPTYRSERASNVALASNLEU
22   HISGLUHISALAGLUALAHISSERGLULEU
23   PROARGGLNALALYS

Samples:

sample_1: Dronpa, [U-99% 13C; U-99% 15N], 2.3 mM; H2O 98%; D2O 2%; phosphate buffer 20 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 96.4 mM; pH: 7.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 17163
PDB
DBJ BAD72874
GB ABV80244 ADE48854

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks