BMRB Entry 17162

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17162

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for a photochromic fluorescent protein Dronpa in the bright state
Deposition date:
2010-09-01
Original release date:
2010-11-09
Authors:
Mizuno, Hideaki; Mal, Tapas; Waelchli, Markus; Fukano, Takashi; Ikura, Mitsuhiko; Miyawaki, Atsushi
Citation:

Citation: Mizuno, Hideaki; Mal, Tapas Kumar; Walchli, Markus; Fukano, Takashi; Ikura, Mitsuhiko; Miyawaki, Atsushi. "Molecular basis of photochromism of a fluorescent protein revealed by direct (13)C detection under laser illumination."  J. Biomol. NMR 48, 237-246 (2010).
PubMed: 21052778

Assembly members:

Assembly members:
Dronpa, polymer, 225 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: stony corals   Taxonomy ID: 301887   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Echinophyllia not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dronpa: GSHMSVIKPDMKIKLRMEGA VNGHPFAIEGVGLGKPFEGK QSMDLKVKEGGPLPFAYDIL TTVFXNRVFAKYPENIVDYF KQSFPEGYSWERSMNYEDGG ICNATNDITLDGDCYIYEIR FDGVNFPANGPVMQKRTVKW EPSTEKLYVRDGVLKGDVNM ALSLEGGGHYRCDFKTTYKA KKVVQLPDYHFVDHHIEIKS HDKDYSNVNLHEHAEAHSEL PRQAK

Data sets:
Data typeCount
13C chemical shifts392
15N chemical shifts196
1H chemical shifts196

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Dronpa1

Entities:

Entity 1, Dronpa 225 residues - Formula weight is not available

1   GLYSERHISMETSERVALILELYSPROASP
2   METLYSILELYSLEUARGMETGLUGLYALA
3   VALASNGLYHISPROPHEALAILEGLUGLY
4   VALGLYLEUGLYLYSPROPHEGLUGLYLYS
5   GLNSERMETASPLEULYSVALLYSGLUGLY
6   GLYPROLEUPROPHEALATYRASPILELEU
7   THRTHRVALPHEGYCASNARGVALPHEALA
8   LYSTYRPROGLUASNILEVALASPTYRPHE
9   LYSGLNSERPHEPROGLUGLYTYRSERTRP
10   GLUARGSERMETASNTYRGLUASPGLYGLY
11   ILECYSASNALATHRASNASPILETHRLEU
12   ASPGLYASPCYSTYRILETYRGLUILEARG
13   PHEASPGLYVALASNPHEPROALAASNGLY
14   PROVALMETGLNLYSARGTHRVALLYSTRP
15   GLUPROSERTHRGLULYSLEUTYRVALARG
16   ASPGLYVALLEULYSGLYASPVALASNMET
17   ALALEUSERLEUGLUGLYGLYGLYHISTYR
18   ARGCYSASPPHELYSTHRTHRTYRLYSALA
19   LYSLYSVALVALGLNLEUPROASPTYRHIS
20   PHEVALASPHISHISILEGLUILELYSSER
21   HISASPLYSASPTYRSERASNVALASNLEU
22   HISGLUHISALAGLUALAHISSERGLULEU
23   PROARGGLNALALYS

Related Database Links:

BMRB 17163
PDB 2GX0 2GX2 2IE2 2IOV 2POX 2Z1O 2Z6X 2Z6Y 2Z6Z 4EMQ 4HQ8 4HQ9 4HQC 4IZN 4UTS
DBJ BAD72874
GB ABV80244 ADE48854

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks