BMRB Entry 17158
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PDB ID: 2l31
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17158
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Eustermann, Sebastian; Videler, Hortense; Yang, Ji-Chun; Cole, Paul; Gruszka, Dominika; Veprintsev, Dmitry; Neuhaus, David. "The DNA-Binding Domain of Human PARP-1 Interacts with DNA Single-Strand Breaks as a Monomer through Its Second Zinc Finger." J. Mol. Biol. 407, 149-170 (2011).
PubMed: 21262234
Assembly members:
PARP-1_Finger_2, polymer, 112 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET28A
Entity Sequences (FASTA):
PARP-1_Finger_2: GSKAEKTLGDFAAEYAKSNR
STCKGCMEKIEKGQVRLSKK
MVDPEKPQLGMIDRWYHPGC
FVKNREELGFRPEYSASQLK
GFSLLATEDKEALKKQLPGV
KSEGKRKGDEVD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 366 |
| 15N chemical shifts | 110 |
| 1H chemical shifts | 693 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PARP-1 Finger 2 | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, PARP-1 Finger 2 112 residues - Formula weight is not available
| 1 | GLY | SER | LYS | ALA | GLU | LYS | THR | LEU | GLY | ASP | ||||
| 2 | PHE | ALA | ALA | GLU | TYR | ALA | LYS | SER | ASN | ARG | ||||
| 3 | SER | THR | CYS | LYS | GLY | CYS | MET | GLU | LYS | ILE | ||||
| 4 | GLU | LYS | GLY | GLN | VAL | ARG | LEU | SER | LYS | LYS | ||||
| 5 | MET | VAL | ASP | PRO | GLU | LYS | PRO | GLN | LEU | GLY | ||||
| 6 | MET | ILE | ASP | ARG | TRP | TYR | HIS | PRO | GLY | CYS | ||||
| 7 | PHE | VAL | LYS | ASN | ARG | GLU | GLU | LEU | GLY | PHE | ||||
| 8 | ARG | PRO | GLU | TYR | SER | ALA | SER | GLN | LEU | LYS | ||||
| 9 | GLY | PHE | SER | LEU | LEU | ALA | THR | GLU | ASP | LYS | ||||
| 10 | GLU | ALA | LEU | LYS | LYS | GLN | LEU | PRO | GLY | VAL | ||||
| 11 | LYS | SER | GLU | GLY | LYS | ARG | LYS | GLY | ASP | GLU | ||||
| 12 | VAL | ASP |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
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