BMRB Entry 17152

Title:
Helical hairpin structure of a novel antimicrobial peptide EcAMP1 from seeds of barnyard grass (Echinochloa crus-galli)
Deposition date:
2010-08-27
Original release date:
2011-05-04
Authors:
Nolde, Svetlana; Barinov, Nikolay; Balashova, Tamara; Arseniev, Alexander
Citation:

Citation: Nolde, Svetlana; Vassilevski, Alexander; Rogozhin, Eugene; Barinov, Nikolay; Balashova, Tamara; Samsonova, Olga; Baranov, Yuri; Feofanov, Alexey; Egorov, Tsezi; Arseniev, Alexander; Grishin, Eugene. "Disulfide-stabilized helical hairpin structure and activity of a novel antifungal peptide EcAMP1 from seeds of barnyard grass (Echinochloa crus-galli)."  J. Biol. Chem. 286, 25145-25153 (2011).
PubMed: 21561864

Assembly members:

Assembly members:
EcAMP1, polymer, 37 residues, 4288.794 Da.

Natural source:

Natural source:   Common Name: Barnyard grass   Taxonomy ID: 90397   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Echinochloa crus-galli

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Echinochloa crusgalli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EcAMP1: GSGRGSCRSQCMRRHEDEPW RVQECVSQCRRRRGGGD

Data sets:
Data typeCount
1H chemical shifts227

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EcAMP11

Entities:

Entity 1, EcAMP1 37 residues - 4288.794 Da.

1   GLYSERGLYARGGLYSERCYSARGSERGLN
2   CYSMETARGARGHISGLUASPGLUPROTRP
3   ARGVALGLNGLUCYSVALSERGLNCYSARG
4   ARGARGARGGLYGLYGLYASP

Samples:

sample_1: EcAMP1 0.7 mg/ml; KOH 10 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 10 mM; pH: 6.62; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AFU90746 AFU90747 AFU90748 AFU90750 AFU90751
SP B3EWR6
AlphaFold B3EWR6