BMRB Entry 17152

Name: Helical hairpin structure of a novel antimicrobial peptide EcAMP1 from seeds of barnyard grass (Echinochloa crus-galli)
Published: 2011-05-04

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PDB ID: 2l2r
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17152
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Helical hairpin structure of a novel antimicrobial peptide EcAMP1 from seeds of barnyard grass (Echinochloa crus-galli)

Deposition date:

2010-08-27

Original release date:

2011-05-04

Authors:

Nolde, Svetlana; Barinov, Nikolay; Balashova, Tamara; Arseniev, Alexander

Citation:

Citation: Nolde, Svetlana; Vassilevski, Alexander; Rogozhin, Eugene; Barinov, Nikolay; Balashova, Tamara; Samsonova, Olga; Baranov, Yuri; Feofanov, Alexey; Egorov, Tsezi; Arseniev, Alexander; Grishin, Eugene. "Disulfide-stabilized helical hairpin structure and activity of a novel antifungal peptide EcAMP1 from seeds of barnyard grass (Echinochloa crus-galli)." J. Biol. Chem. 286, 25145-25153 (2011).
PubMed: 21561864

Assembly members:

Assembly members:
EcAMP1, polymer, 37 residues, 4288.794 Da.

Natural source:

Natural source: Common Name: Barnyard grass Taxonomy ID: 90397 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Echinochloa crus-galli

Experimental source:

Experimental source: Production method: purified from the natural source Host organism: Echinochloa crusgalli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EcAMP1: GSGRGSCRSQCMRRHEDEPW RVQECVSQCRRRRGGGD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	227

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EcAMP1	1

Entities:

Entity 1, EcAMP1 37 residues - 4288.794 Da.

1

GLY

SER

GLY

ARG

GLY

SER

CYS

ARG

SER

GLN

2

CYS

MET

ARG

HIS

GLU

ASP

GLU

PRO

TRP

3

ARG

VAL

GLN

GLU

CYS

VAL

SER

GLN

CYS

ARG

4

ARG

GLY

ASP

Samples:

sample_1: EcAMP1 0.7 mg/ml; KOH 10 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 10 mM; pH: 6.62; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Bruker Avance 800 MHz

PDB	2L2R
GB	AFU90746 AFU90747 AFU90748 AFU90750 AFU90751
SP	B3EWR6
AlphaFold	B3EWR6