BMRB Entry 17148

Name: N-terminal domain of Nephila clavipes major ampulate spidroin 1
Published: 2010-09-13

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17148

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

N-terminal domain of Nephila clavipes major ampulate spidroin 1

Deposition date:

2010-08-25

Original release date:

2010-09-13

Authors:

Parnham, Stuart; Hennig, Mirko

Citation:

Citation: Parnham, Stuart; Gaines, William; Duggan, Brendan; Marcotte, William; Hennig, Mirko. "NMR assignments of the N-terminal domain of Nephila clavipes spidroin 1." Biomol. NMR Assignments 5, 131-133 (2011).
PubMed: 21152998

Assembly members:

Assembly members:
Spn-1, polymer, 144 residues, 14961.5253 Da.

Natural source:

Natural source: Common Name: Nephila clavipes Taxonomy ID: 6915 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Nephila clavipes

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: GinG

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Spn-1: GPLGSPGIPGQNTPWSSTEL ADAFINAFMNEAGRTGAFTA DQLDDMSTIGDTIKTAMDKM ARSNKSSKGKLQALNMAFAS SMAEIAAVEQGGLSVDAKTN AIADSLNSAFYQTTGAANPQ FVNEIRSLINMFAQSSANEV SYGG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
coupling_constants
- coupling_constant_list_1
spectral_peak_list

Data type	Count
13C chemical shifts	539
15N chemical shifts	160
1H chemical shifts	872
coupling constants	98

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Spn-1	1

Entities:

Entity 1, Spn-1 144 residues - 14961.5253 Da.

1

GLY

PRO

LEU

GLY

SER

PRO

GLY

ILE

PRO

GLY

2

GLN

ASN

THR

PRO

TRP

SER

THR

GLU

LEU

3

ALA

ASP

ALA

PHE

ILE

ASN

ALA

PHE

MET

ASN

4

GLU

ALA

GLY

ARG

THR

GLY

ALA

PHE

THR

ALA

5

ASP

GLN

LEU

ASP

MET

SER

THR

ILE

GLY

6

ASP

THR

ILE

LYS

THR

ALA

MET

ASP

LYS

MET

7

ALA

ARG

SER

ASN

LYS

SER

LYS

GLY

LYS

8

LEU

GLN

ALA

LEU

ASN

MET

ALA

PHE

ALA

SER

9

SER

MET

ALA

GLU

ILE

ALA

VAL

GLU

GLN

10

GLY

LEU

SER

VAL

ASP

ALA

LYS

THR

ASN

11

ALA

ILE

ALA

ASP

SER

LEU

ASN

SER

ALA

PHE

12

TYR

GLN

THR

GLY

ALA

ASN

PRO

GLN

13

PHE

VAL

ASN

GLU

ILE

ARG

SER

LEU

ILE

ASN

14

MET

PHE

ALA

GLN

SER

ALA

ASN

GLU

VAL

15

SER

TYR

GLY

Name	Sample	Sample state	Sample conditions
aro_d20noesy (H[c]_H[c].NOESY)	Spn-1	isotropic	sample_conditions_1
3D C(CO)NH	Spn-1	isotropic	sample_conditions_1
2D 1H-15N HSQC/HMQC	Spn-1	isotropic	sample_conditions_1
cbca_500mM (H[N[co[{CA\|ca[C]}]]])	Spn-1	isotropic	sample_conditions_1
3D 1H-15N NOESY	Spn-1	isotropic	sample_conditions_1
3D 1H-13C NOESY	Spn-1	isotropic	sample_conditions_1
3D H(CCO)NH	Spn-1	isotropic	sample_conditions_1
500mM_aro (h[C]_H.TOCSY)	Spn-1	isotropic	sample_conditions_1
HCaN_prolines (HCA[N])	Spn-1	isotropic	sample_conditions_1
hnco_500mM (HNCO)	Spn-1	isotropic	sample_conditions_1
hnha (H{[N]+[HA]})	Spn-1	isotropic	sample_conditions_1
hbhaconh_500 (H{ca\|cca}[co[N]]HA)	Spn-1	isotropic	sample_conditions_1

BMRB Entry 17148

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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