BMRB Entry 17143

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PDB ID: 2l2n
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17143
MolProbity Validation Chart

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the first dsRBD of protein HYL1
Deposition date:
2010-08-23
Original release date:
2010-09-24
Authors:
Rasia, Rodolfo; Mateos, Julieta; Bologna, Nicolas; Burdisso, Paula; Imbert, Lionel; Palatnik, Javier; Boisbouvier, Jerome
Citation:

Citation: Rasia, Rodolfo; Mateos, Julieta; Bologna, Nicolas; Burdisso, Paula; Imbert, Lionel; Palatnik, Javier; Boisbouvier, Jerome. "Structure and RNA Interactions of the Plant MicroRNA Processing-Associated Protein HYL1."  Biochemistry 49, 8237-8239 (2010).
PubMed: 20735118

Assembly members:

Assembly members:
HYL1_dsRBD_1, polymer, 103 residues, 8031.225 Da.

Natural source:

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-TEV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HYL1_dsRBD_1: GHMMTSTDVSSGVSNCYVFK SRLQEYAQKYKLPTPVYEIV KEGPSHKSLFQSTVILDGVR YNSLPGFFNRKAAEQSAAEV ALRELAKSSELSQCVSQPVH ETG

Data sets:
Data typeCount
13C chemical shifts264
15N chemical shifts80
1H chemical shifts156
residual dipolar couplings292

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1HYL1_dsRBD_1-11

Entities:

Entity 1, HYL1_dsRBD_1-1 103 residues - 8031.225 Da.

1   GLYHISMETMETTHRSERTHRASPVALSER
2   SERGLYVALSERASNCYSTYRVALPHELYS
3   SERARGLEUGLNGLUTYRALAGLNLYSTYR
4   LYSLEUPROTHRPROVALTYRGLUILEVAL
5   LYSGLUGLYPROSERHISLYSSERLEUPHE
6   GLNSERTHRVALILELEUASPGLYVALARG
7   TYRASNSERLEUPROGLYPHEPHEASNARG
8   LYSALAALAGLUGLNSERALAALAGLUVAL
9   ALALEUARGGLULEUALALYSSERSERGLU
10   LEUSERGLNCYSVALSERGLNPROVALHIS
11   GLUTHRGLY

Related Database Links:

PDB 2L2N 3ADG 3ADI
GB AAB60726 AAG49890 AAK96822 AAM10087 AEE28481
REF NP_563850
SP O04492
AlphaFold O04492

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks