BMRB Entry 17141

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17141

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for HYL1-dsRBD2
Deposition date:
2010-08-22
Original release date:
2010-09-28
Authors:
Rasia, Rodolfo; Mateos, Julieta; Bologna, Nicolas; Burdisso, Paula; Imbert, Lionel; Palatnik, Javier; Boisbouvier, Jerome
Citation:

Citation: Rasia, Rodolfo; Mateos, Julieta; Bologna, Nicolas; Burdisso, Paula; Imbert, Lionel; Palatnik, Javier; Boisbouvier, Jerome. "Structure and RNA Interactions of the Plant MicroRNA Processing-Associated Protein HYL1."  Biochemistry 49, 8237-8239 (2010).
PubMed: 20735118

Assembly members:

Assembly members:
hyl1-2, polymer, 77 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-TEV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hyl1-2: GHMHETGLCKNLLQEYAQKM NYAIPLYQCQKVETLGRVTQ FTCTVEIGGIKYTGAATRTK KDAEISAGRTALLAIQS

Data sets:
Data typeCount
13C chemical shifts195
15N chemical shifts63
1H chemical shifts63
residual dipolar couplings181

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HYL1 second dsRBD1

Entities:

Entity 1, HYL1 second dsRBD 77 residues - Formula weight is not available

1   GLYHISMETHISGLUTHRGLYLEUCYSLYS
2   ASNLEULEUGLNGLUTYRALAGLNLYSMET
3   ASNTYRALAILEPROLEUTYRGLNCYSGLN
4   LYSVALGLUTHRLEUGLYARGVALTHRGLN
5   PHETHRCYSTHRVALGLUILEGLYGLYILE
6   LYSTYRTHRGLYALAALATHRARGTHRLYS
7   LYSASPALAGLUILESERALAGLYARGTHR
8   ALALEULEUALAILEGLNSER

Samples:

sample_1: hyl1-2, [U-100% 13C; U-100% 15N], 0.8 mM; DTT 10 mM; sodium phosphate 100 mM; sodium chloride 50 mM; sodium azide 0.05%; H2O 90%; D2O 10%

sample_aligned: hyl1-2, [U-100% 13C; U-100% 15N], 0.8 mM; DTT 10 mM; sodium phosphate 100 mM; sodium chloride 50 mM; sodium azide 0.05%; C12E5 5%; Hexanol 0.2%; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCO JCACOsample_1isotropicsample_conditions_1
3D HNCO JNHsample_1isotropicsample_conditions_1
3D HNCOCA JCAHAsample_1isotropicsample_conditions_1
3D HNCO JCACOsample_alignedanisotropicsample_conditions_1
3D HNCO JNHsample_alignedanisotropicsample_conditions_1
3D HNCOCA JCAHAsample_alignedanisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Varian DirectDrive 600 MHz

Related Database Links:

PDB
DBJ BAH57238
GB AAB60726 AAG49890 AAK96822 AAM10087 AEE28481
REF NP_563850
SP O04492
AlphaFold O04492

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks