BMRB Entry 17136

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17136

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Title:
Rhodanese G
Deposition date:
2010-08-20
Original release date:
2010-10-26
Authors:
Li, Hongwei; Jin, Changwen
Citation:

Citation: Li, Hongwei; Xia, Bin; Jin, Changwen. "(1)H, (13)C and (15)N resonance assignments of rhodanese GlpE from Escherichia coli."  Biomol. NMR Assignments 5, 97-99 (2011).
PubMed: 20960079

Assembly members:

Assembly members:
GlpE, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GlpE: MDQFECINVADAHQKLQEKE AVLVDIRDPQSFAMGHAVQA FHLTNDTLGAFMRDNDFDTP VMVMCYHGNSSKGAAQYLLQ QGYDVVYSIDGGFEAWQRQF PAEVAYGA

Data sets:
Data typeCount
13C chemical shifts302
15N chemical shifts120
1H chemical shifts671

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GlpE1

Entities:

Entity 1, GlpE 108 residues - Formula weight is not available

1   METASPGLNPHEGLUCYSILEASNVALALA
2   ASPALAHISGLNLYSLEUGLNGLULYSGLU
3   ALAVALLEUVALASPILEARGASPPROGLN
4   SERPHEALAMETGLYHISALAVALGLNALA
5   PHEHISLEUTHRASNASPTHRLEUGLYALA
6   PHEMETARGASPASNASPPHEASPTHRPRO
7   VALMETVALMETCYSTYRHISGLYASNSER
8   SERLYSGLYALAALAGLNTYRLEULEUGLN
9   GLNGLYTYRASPVALVALTYRSERILEASP
10   GLYGLYPHEGLUALATRPGLNARGGLNPHE
11   PROALAGLUVALALATYRGLYALA

Samples:

sample_1: GlpE, [U-99% 13C; U-99% 15N], 1 mM; TRIS 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAB37691 BAE77867 BAG79215 BAI27682 BAI32851
EMBL CAA30397 CAP77870 CAQ33745 CAQ90870 CAR00367
GB AAA23889 AAA58223 AAC28165 AAC76450 AAN44907
REF NP_312295 NP_417883 NP_709200 WP_000362869 WP_000371922
SP A1AGU8 A7ZSV5 A8A5N3 B1IP41 B1LI85
AlphaFold A1AGU8 A7ZSV5 A8A5N3 B1IP41 B1LI85

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks