BMRB Entry 17135

Title:
Mature Drosophila Frataxin Homolog (Dfh)
Deposition date:
2010-08-18
Original release date:
2014-03-07
Authors:
Rawat, swati; Stemmler, Timothy
Citation:

Citation: Rawat, swati; Stemmler, Timothy. "Backbone Atom Chemical Shift Assignments for mature Drosophila Frataxin Homolog (Dfh)"  J. Biomol. NMR ., .-..

Assembly members:

Assembly members:
Drosophila_Frataxin_homolg, polymer, 133 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET101/TOPO

Data sets:
Data typeCount
13C chemical shifts249
15N chemical shifts127
1H chemical shifts126

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Drosophila Frataxin Homolg1

Entities:

Entity 1, Drosophila Frataxin Homolg 133 residues - Formula weight is not available

After removing the mitochondrial targeting sequence that is initial 58 residues, tha mature protein starts with the 59-190 residues. We have assigned 128 out of 133 residues for mature Dfh.

1   METSERSERGLNILEGLUTHRGLUSERTHR
2   LEUASPGLYALATHRTYRGLUARGVALCYS
3   SERASPTHRLEUASPALALEUCYSASPTYR
4   PHEGLUGLULEUTHRGLUASNALASERGLU
5   LEUGLNGLYTHRASPVALALATYRSERASP
6   GLYVALLEUTHRVALASNLEUGLYGLYGLN
7   HISGLYTHRTYRVALILEASNARGGLNTHR
8   PROASNLYSGLNILETRPLEUSERSERPRO
9   THRSERGLYPROLYSARGTYRASPPHEVAL
10   GLYTHRVALALAALAGLYARGTRPILETYR
11   LYSHISSERGLYGLNSERLEUHISGLULEU
12   LEUGLNGLNGLUILEPROGLYILELEULYS
13   SERGLNSERVALASPPHELEUARGLEUPRO
14   TYRCYSSER

Samples:

sample_1: HEPES 20 mM; sodium chloride 150 mM; Drosophila Frataxin, [U-13C; U-15N], 1 mM; H2O 93%; D2O 7%

Sample_conditions: pH: 7.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicSample_conditions
2D 1H-13C HSQCsample_1isotropicSample_conditions
3D HNCACBsample_1isotropicSample_conditions
3D CBCA(CO)NHsample_1isotropicSample_conditions
3D HNCOsample_1isotropicSample_conditions

Software:

NMRView vJ, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - chemical shift assignment, processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian UnityPlus 720 MHz

Related Database Links:

EMBL CAC20098
GB AAF46449 AAG35732 AAG35733 AAM11002 ACL87329
REF NP_511094 XP_002043984 XP_002106519
SP Q9W385
AlphaFold Q9W385

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks