BMRB Entry 17135

Name: Mature Drosophila Frataxin Homolog (Dfh)
Published: 2014-03-07

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17135

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Mature Drosophila Frataxin Homolog (Dfh)

Deposition date:

2010-08-18

Original release date:

2014-03-07

Authors:

Rawat, swati; Stemmler, Timothy

Citation:

Citation: Rawat, swati; Stemmler, Timothy. "Backbone Atom Chemical Shift Assignments for mature Drosophila Frataxin Homolog (Dfh)" J. Biomol. NMR ., .-..

Assembly members:

Assembly members:
Drosophila_Frataxin_homolg, polymer, 133 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET101/TOPO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Drosophila_Frataxin_homolg: MSSQIETESTLDGATYERVC SDTLDALCDYFEELTENASE LQGTDVAYSDGVLTVNLGGQ HGTYVINRQTPNKQIWLSSP TSGPKRYDFVGTVAAGRWIY KHSGQSLHELLQQEIPGILK SQSVDFLRLPYCS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	249
15N chemical shifts	127
1H chemical shifts	126

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Drosophila Frataxin Homolg	1

Entities:

Entity 1, Drosophila Frataxin Homolg 133 residues - Formula weight is not available

After removing the mitochondrial targeting sequence that is initial 58 residues, tha mature protein starts with the 59-190 residues. We have assigned 128 out of 133 residues for mature Dfh.

1

MET

SER

GLN

ILE

GLU

THR

GLU

SER

THR

2

LEU

ASP

GLY

ALA

THR

TYR

GLU

ARG

VAL

CYS

3

SER

ASP

THR

LEU

ASP

ALA

LEU

CYS

ASP

TYR

4

PHE

GLU

LEU

THR

GLU

ASN

ALA

SER

GLU

5

LEU

GLN

GLY

THR

ASP

VAL

ALA

TYR

SER

ASP

6

GLY

VAL

LEU

THR

VAL

ASN

LEU

GLY

GLN

7

HIS

GLY

THR

TYR

VAL

ILE

ASN

ARG

GLN

THR

8

PRO

ASN

LYS

GLN

ILE

TRP

LEU

SER

PRO

9

THR

SER

GLY

PRO

LYS

ARG

TYR

ASP

PHE

VAL

10

GLY

THR

VAL

ALA

GLY

ARG

TRP

ILE

TYR

11

LYS

HIS

SER

GLY

GLN

SER

LEU

HIS

GLU

LEU

12

LEU

GLN

GLU

ILE

PRO

GLY

ILE

LEU

LYS

13

SER

GLN

SER

VAL

ASP

PHE

LEU

ARG

LEU

PRO

14

TYR

CYS

SER

Samples:

sample_1: HEPES 20 mM; sodium chloride 150 mM; Drosophila Frataxin, [U-13C; U-15N], 1 mM; H2O 93%; D2O 7%

Sample_conditions: pH: 7.5; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	Sample_conditions
2D 1H-13C HSQC	sample_1	isotropic	Sample_conditions
3D HNCACB	sample_1	isotropic	Sample_conditions
3D CBCA(CO)NH	sample_1	isotropic	Sample_conditions
3D HNCO	sample_1	isotropic	Sample_conditions

Software:

NMRView vJ, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - chemical shift assignment, processing

NMR spectrometers:

Varian INOVA 600 MHz
Varian UnityPlus 720 MHz

EMBL	CAC20098
GB	AAF46449 AAG35732 AAG35733 AAM11002 ACL87329
REF	NP_511094 XP_002043984 XP_002106519
SP	Q9W385
AlphaFold	Q9W385