BMRB Entry 17123
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17123
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Citation: Wijesinha-Bettoni, Ramani; Dobson, Christopher; Redfield, Christina. "Comparison of the Structural and Dynamical Properties of Holo and Apo Bovine alpha-Lactalbumin by NMR Spectroscopy" J. Mol. Biol. 307, 885-898 (2001).
PubMed: 11273708
Assembly members:
alphaL, polymer, 123 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
Natural source: Common Name: cattle Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
alphaL: EQLTKCEVFRELKDLKGYGG
VSLPEWVCTTFHTSGYDTQA
IVQNNDSTEYGLFQINNKIW
CKDDQNPHSSNICNISCDKF
LDDDLTDDIMCVKKILDKVG
INYWLAHKALCSEKLDQWLC
EKL
- chemical_rates
| Data type | Count |
| kinetic rates | 3 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | alphaL | 1 |
| 2 | CA | 2 |
Entities:
Entity 1, alphaL 123 residues - Formula weight is not available
| 1 | GLU | GLN | LEU | THR | LYS | CYS | GLU | VAL | PHE | ARG | ||||
| 2 | GLU | LEU | LYS | ASP | LEU | LYS | GLY | TYR | GLY | GLY | ||||
| 3 | VAL | SER | LEU | PRO | GLU | TRP | VAL | CYS | THR | THR | ||||
| 4 | PHE | HIS | THR | SER | GLY | TYR | ASP | THR | GLN | ALA | ||||
| 5 | ILE | VAL | GLN | ASN | ASN | ASP | SER | THR | GLU | TYR | ||||
| 6 | GLY | LEU | PHE | GLN | ILE | ASN | ASN | LYS | ILE | TRP | ||||
| 7 | CYS | LYS | ASP | ASP | GLN | ASN | PRO | HIS | SER | SER | ||||
| 8 | ASN | ILE | CYS | ASN | ILE | SER | CYS | ASP | LYS | PHE | ||||
| 9 | LEU | ASP | ASP | ASP | LEU | THR | ASP | ASP | ILE | MET | ||||
| 10 | CYS | VAL | LYS | LYS | ILE | LEU | ASP | LYS | VAL | GLY | ||||
| 11 | ILE | ASN | TYR | TRP | LEU | ALA | HIS | LYS | ALA | LEU | ||||
| 12 | CYS | SER | GLU | LYS | LEU | ASP | GLN | TRP | LEU | CYS | ||||
| 13 | GLU | LYS | LEU |
Entity 2, CA - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: alphaL 1.4 mM; sodium phosphate buffer 15 mM; EDTA 25 mM; sodium chloride 0.5 mM; deuterated imidazole buffer 100 mM; CaCl2 2.8 mM; H2O 100%
sample_conditions_1: pH: 7.2; pressure: ambient atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H Real Time NMR | sample_1 | isotropic | sample_conditions_1 |
Software:
TOTAL, (http:// www.shef.ac.uk/ nmr/resources.html - ring current calculation
NMR spectrometers:
- Home Built na 500 MHz