BMRB Entry 17115

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17115

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Title:
Effects of Domain Dissection on the Folding and Stability of the 43 kDa Protein PGK Probed by NMR
Deposition date:
2010-08-11
Original release date:
2010-11-10
Authors:
Reed, Michelle; Hounslow, Andrea; Sze, K.; Barsukov, Igor; Hosszu, Laszlo; Clarke, Anthony; Craven, C.; Waltho, Jonathan
Citation:

Citation: Reed, Michelle; Hounslow, Andrea; Sze, K.; Barsukov, Igor; Hosszu, Laszlo; Clarke, Anthony; Craven, C.; Waltho, Jonathan. "Effects of Domain Dissection on the Folding and Stability of the 43 kDa Protein PGK Probed by NMR"  J. Mol. Biol. 330, 1189-1201 (2003).
PubMed: 12860138

Assembly members:

Assembly members:
PGK, polymer, 394 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Geobacillus stearothermophilus   Taxonomy ID: 1422   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Geobacillus stearothermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKK223-3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PGK: MNKKTIRDVDVRGKRVFCRV DFNVPMEQGAITDDTRIRAA LPTIRYLIEHGAKVILASHL GRPKGKVVEELRLDAVAKRL GELLERPVAKTNEAVGDEVK AAVDRLNEGDVLLLENVRFY PGEEKNDPELAKAFAELADL YVNDAFGAAHRAHASTEGIA HYLPAVAGFLMEKELEVLGK ALSNPDRPFTAIIGGAKVKD KIGVIDNLLEKVDNLIIGGG LAYTFVKALGHDVGKSLLEE DKIELAKSFMEKAKEKGVRF YMPVDVVVADRFANDANTKV VPIDAIPADYSALDIGPKTR ELYRDVIRESKLVVWNGPMG VFEMDAFAHGTKAIAEALAE ALDTYSVIGGGDSAAAVEKF GLADKMDHISTGGGASLEFM EGKQLPGVVALEDK

Data sets:
Data typeCount
kinetic rates184

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PGK1

Entities:

Entity 1, PGK 394 residues - Formula weight is not available

1   METASNLYSLYSTHRILEARGASPVALASP
2   VALARGGLYLYSARGVALPHECYSARGVAL
3   ASPPHEASNVALPROMETGLUGLNGLYALA
4   ILETHRASPASPTHRARGILEARGALAALA
5   LEUPROTHRILEARGTYRLEUILEGLUHIS
6   GLYALALYSVALILELEUALASERHISLEU
7   GLYARGPROLYSGLYLYSVALVALGLUGLU
8   LEUARGLEUASPALAVALALALYSARGLEU
9   GLYGLULEULEUGLUARGPROVALALALYS
10   THRASNGLUALAVALGLYASPGLUVALLYS
11   ALAALAVALASPARGLEUASNGLUGLYASP
12   VALLEULEULEUGLUASNVALARGPHETYR
13   PROGLYGLUGLULYSASNASPPROGLULEU
14   ALALYSALAPHEALAGLULEUALAASPLEU
15   TYRVALASNASPALAPHEGLYALAALAHIS
16   ARGALAHISALASERTHRGLUGLYILEALA
17   HISTYRLEUPROALAVALALAGLYPHELEU
18   METGLULYSGLULEUGLUVALLEUGLYLYS
19   ALALEUSERASNPROASPARGPROPHETHR
20   ALAILEILEGLYGLYALALYSVALLYSASP
21   LYSILEGLYVALILEASPASNLEULEUGLU
22   LYSVALASPASNLEUILEILEGLYGLYGLY
23   LEUALATYRTHRPHEVALLYSALALEUGLY
24   HISASPVALGLYLYSSERLEULEUGLUGLU
25   ASPLYSILEGLULEUALALYSSERPHEMET
26   GLULYSALALYSGLULYSGLYVALARGPHE
27   TYRMETPROVALASPVALVALVALALAASP
28   ARGPHEALAASNASPALAASNTHRLYSVAL
29   VALPROILEASPALAILEPROALAASPTYR
30   SERALALEUASPILEGLYPROLYSTHRARG
31   GLULEUTYRARGASPVALILEARGGLUSER
32   LYSLEUVALVALTRPASNGLYPROMETGLY
33   VALPHEGLUMETASPALAPHEALAHISGLY
34   THRLYSALAILEALAGLUALALEUALAGLU
35   ALALEUASPTHRTYRSERVALILEGLYGLY
36   GLYASPSERALAALAALAVALGLULYSPHE
37   GLYLEUALAASPLYSMETASPHISILESER
38   THRGLYGLYGLYALASERLEUGLUPHEMET
39   GLUGLYLYSGLNLEUPROGLYVALVALALA
40   LEUGLUASPLYS

Samples:

sample_1: PGK, [U-2H,U-15N], 0.1 mg/ml; potassium phosphate 20 mM; sodium azide 3 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 7.50; pressure: ambient atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H-15N TROSYsample_1isotropicsample_conditions_1

Software:

FELIX, Accelrys Software Inc. - peak height extraction

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 16447 16451 16464
PDB
DBJ BAD77342 GAD14836 GAJ57507
EMBL CAA41093
GB ACX79693 ADI28008 ADU95522 AEV20724 AGE23620
REF WP_011232527 WP_013146368 WP_013524587 WP_015375782 WP_023633686
SP P18912 Q5KVE4
AlphaFold P18912 Q5KVE4