BMRB Entry 17114
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17114
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Citation: Hansen, D.; Zhou, Zheng; Feng, Haniqiao; Jenkins, Lisa; Bai, Yawen; Kay, Lewis. "Binding Kinetics of Histone Chaperone Chz1 and Variant Histone H2A.Z-H2B by Relaxation Dispersion NMR Spectroscopy" J. Mol. Biol. 387, 1-9 (2009).
PubMed: 19385041
Assembly members:
H2AZH2B, polymer, 192 residues, Formula weight is not available
Chz1, polymer, 62 residues, Formula weight is not available
Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: na
Entity Sequences (FASTA):
H2AZH2B: RKETYSSYIYKVLKQTHPDT
GISQKSMSILNSFVNDIFER
IATEASKLAAYNKKSTISAR
EIQTAVRLILPGELAKHAVS
EGTRAVTKYSSSTQAQSSSA
RAGLQFPVGRIKRYLKRHAT
GRTRVGSKAAIYLTAVLEYL
TAEVLELAGNAAKDLKVKRI
TPRHLQLAIRGDDELDSLIR
ATIASGGVLPHI
Chz1: TVEDSESDMDDAKLDALMGN
EGEEEEDDLAEIDTSNIITS
GRRTRGKVIDYKKTAEELDK
KE
- chemical rates
| Data type | Count |
| binding constants | 2 |
| kinetic rates | 3 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | H2AZH2B | 1 |
| 2 | Chz1 | 2 |
Entities:
Entity 1, H2AZH2B 192 residues - Formula weight is not available
| 1 | ARG | LYS | GLU | THR | TYR | SER | SER | TYR | ILE | TYR | ||||
| 2 | LYS | VAL | LEU | LYS | GLN | THR | HIS | PRO | ASP | THR | ||||
| 3 | GLY | ILE | SER | GLN | LYS | SER | MET | SER | ILE | LEU | ||||
| 4 | ASN | SER | PHE | VAL | ASN | ASP | ILE | PHE | GLU | ARG | ||||
| 5 | ILE | ALA | THR | GLU | ALA | SER | LYS | LEU | ALA | ALA | ||||
| 6 | TYR | ASN | LYS | LYS | SER | THR | ILE | SER | ALA | ARG | ||||
| 7 | GLU | ILE | GLN | THR | ALA | VAL | ARG | LEU | ILE | LEU | ||||
| 8 | PRO | GLY | GLU | LEU | ALA | LYS | HIS | ALA | VAL | SER | ||||
| 9 | GLU | GLY | THR | ARG | ALA | VAL | THR | LYS | TYR | SER | ||||
| 10 | SER | SER | THR | GLN | ALA | GLN | SER | SER | SER | ALA | ||||
| 11 | ARG | ALA | GLY | LEU | GLN | PHE | PRO | VAL | GLY | ARG | ||||
| 12 | ILE | LYS | ARG | TYR | LEU | LYS | ARG | HIS | ALA | THR | ||||
| 13 | GLY | ARG | THR | ARG | VAL | GLY | SER | LYS | ALA | ALA | ||||
| 14 | ILE | TYR | LEU | THR | ALA | VAL | LEU | GLU | TYR | LEU | ||||
| 15 | THR | ALA | GLU | VAL | LEU | GLU | LEU | ALA | GLY | ASN | ||||
| 16 | ALA | ALA | LYS | ASP | LEU | LYS | VAL | LYS | ARG | ILE | ||||
| 17 | THR | PRO | ARG | HIS | LEU | GLN | LEU | ALA | ILE | ARG | ||||
| 18 | GLY | ASP | ASP | GLU | LEU | ASP | SER | LEU | ILE | ARG | ||||
| 19 | ALA | THR | ILE | ALA | SER | GLY | GLY | VAL | LEU | PRO | ||||
| 20 | HIS | ILE |
Entity 2, Chz1 62 residues - Formula weight is not available
| 1 | THR | VAL | GLU | ASP | SER | GLU | SER | ASP | MET | ASP | ||||
| 2 | ASP | ALA | LYS | LEU | ASP | ALA | LEU | MET | GLY | ASN | ||||
| 3 | GLU | GLY | GLU | GLU | GLU | GLU | ASP | ASP | LEU | ALA | ||||
| 4 | GLU | ILE | ASP | THR | SER | ASN | ILE | ILE | THR | SER | ||||
| 5 | GLY | ARG | ARG | THR | ARG | GLY | LYS | VAL | ILE | ASP | ||||
| 6 | TYR | LYS | LYS | THR | ALA | GLU | GLU | LEU | ASP | LYS | ||||
| 7 | LYS | GLU |
Samples:
sample_1: H2AZH2B, [U-15N; U-2H], 0.95 mM; Chz1, [U-15N; U-2H], 1 mM; Mes 25 mM; sodium chloride 0.2 M; EDTA 1 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 6.0; pressure: ambient atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
FuDA, flemming@pound.med.utoronto.ca; http://pound.med.utoronto.ca/software - signal quantification
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz