BMRB Entry 17113

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17113

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Title:
1H, 13C and 15N NMR Assignments of the C1A Subdomains of PKC-delta.
Deposition date:
2010-08-10
Original release date:
2010-12-08
Authors:
Ziemba, Brian; Jones, David
Citation:

Citation: Brian, P. Ziemba; Jamie, C. Booth; David, Jones. "1H, 13C and 15N NMR assignments of the C1A and C1B subdomains of PKC-delta."  Biomol. NMR Assignments 5, 125-129 (2011).
PubMed: 21132404

Assembly members:

Assembly members:
PKC_delta_C1A, polymer, 80 residues, 8977.2 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Norway Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PKC_delta_C1A: GSPEFHYIKNHEFIATFFGQ PTFCSVCKEFVWGLNKQGYK CRQCNAAIHKKCIDKIIGRC TGTATNSRDTVDSSGRIVTD

Data sets:
Data typeCount
13C chemical shifts295
15N chemical shifts72
1H chemical shifts439

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C1A construct1
2Zinc_12
3Zinc_22

Entities:

Entity 1, C1A construct 80 residues - 8977.2 Da.

The C1A construct contains a total of 80 amino acids (labeled 149-228) derived from PKC-delta (residues 154-218) including the C1A domain (residues 159-208) and residues from the pGEX-4T1 vector (residues 149-153 and 219-228).

1   GLYSERPROGLUPHEHISTYRILELYSASN
2   HISGLUPHEILEALATHRPHEPHEGLYGLN
3   PROTHRPHECYSSERVALCYSLYSGLUPHE
4   VALTRPGLYLEUASNLYSGLNGLYTYRLYS
5   CYSARGGLNCYSASNALAALAILEHISLYS
6   LYSCYSILEASPLYSILEILEGLYARGCYS
7   THRGLYTHRALATHRASNSERARGASPTHR
8   VALASPSERSERGLYARGILEVALTHRASP

Entity 2, Zinc_1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: D2O, [U-100% 2H], 10%; DTT 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; zinc chloride 10 uM; H2O 90%; PKC_delta_C1A, [U-98% 15N; U-99% 13C], 300 uM

sample_2: D2O, [U-2H], 100%; DTT 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; zinc chloride 10 uM; PKC_delta_C1A, [U-98% 15N; U-99% 13C], 300 uM

sample_conditions_1: pH: 6.9; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
2D (HB)CB(CGCDCE)HEsample_2isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_2isotropicsample_conditions_1

Software:

NMRDraw v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

ANALYSIS v1.0, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 900 MHz
  • Varian INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks