BMRB Entry 17112

Name: 1H, 13C and 15N NMR Assignments of the C1B Subdomains of PKC-delta.
Published: 2010-12-08

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17112

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N NMR Assignments of the C1B Subdomains of PKC-delta.

Deposition date:

2010-08-10

Original release date:

2010-12-08

Authors:

Ziemba, Brian; Jones, David; Booth, Jamie

Citation:

Citation: Ziemba, Brian; Booth, Jamie; Jones, David. "1H, 13C and 15N NMR assignments of the C1A and C1B subdomains of PKC-delta." Biomol. NMR Assignments 5, 125-129 (2011).
PubMed: 21132404

Assembly members:

Assembly members:
PKC_delta_C1B, polymer, 82 residues, 9408.8 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PKC_delta_C1B: GSPEFIFQKERFNIDMPHRF KVYNYMSPTFCDHCGSLLWG LVKQGLKCEDCGMNVHHKCR EKVANLCGINQKVDSSGRIV TD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	319
15N chemical shifts	73
1H chemical shifts	441

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C1B construct	1
2	Zinc 1	2
3	Zinc 2	2

Entities:

Entity 1, C1B construct 82 residues - 9408.8 Da.

Residues 214-218 and 286-295 are derived from pGEX-4T1 vector. Residues 219-285 are from PKC-delta, and 231-280 is the 50 residue C1B domain.

1

GLY

SER

PRO

GLU

PHE

ILE

PHE

GLN

LYS

GLU

2

ARG

PHE

ASN

ILE

ASP

MET

PRO

HIS

ARG

PHE

3

LYS

VAL

TYR

ASN

TYR

MET

SER

PRO

THR

PHE

4

CYS

ASP

HIS

CYS

GLY

SER

LEU

TRP

GLY

5

LEU

VAL

LYS

GLN

GLY

LEU

LYS

CYS

GLU

ASP

6

CYS

GLY

MET

ASN

VAL

HIS

LYS

CYS

ARG

7

GLU

LYS

VAL

ALA

ASN

LEU

CYS

GLY

ILE

ASN

8

GLN

LYS

VAL

ASP

SER

GLY

ARG

ILE

VAL

9

THR

ASP

Entity 2, Zinc 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: PKC_delta_C1B, [U-98% 15N; U-99% 13C], 300 uM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 1 mM; zinc chloride 10 uM; D2O, [U-100% 2H], 10%; H2O, [U-100% 2H], 90%

sample_2: PKC_delta_C1B, [U-98% 15N; U-99% 13C], 300 uM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 1 mM; zinc chloride 10 uM; D2O, [U-100% 2H], 100%

sample_conditions_1: pH: 6.9; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
2D (HB)CB(CGCD)HD	sample_2	isotropic	sample_conditions_1
2D (HB)CB(CGCDCE)HE	sample_2	isotropic	sample_conditions_1

Software:

NMRDraw v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

ANALYSIS v1.0, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Varian INOVA 900 MHz
Varian INOVA 600 MHz