BMRB Entry 17098

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17098

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Title:
Cofactor fingerprinting with STD NMR to characterize proteins of unknown function: identification of a rare cCMP cofactor preference
Deposition date:
2010-08-02
Original release date:
2010-11-10
Authors:
Yao, Huili; Sem, Daniel
Citation:

Citation: Yao, Huili; Sem, Daniel. "Cofactor fingerprinting with STD NMR to characterize proteins of unknown function: identification of a rare cCMP cofactor preference"  FEBS Lett. 579, 661-666 (2005).
PubMed: 15670825

Assembly members:

Assembly members:
RSP2, polymer, 738 residues, 507.181 Da.
ATP, non-polymer, 507.181 Da.

Natural source:

Natural source:   Common Name: Green algae   Taxonomy ID: 3055   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Chlamydomonas reinhardtii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RSP2: MAPTQAGHDTAYLKETVGEA LARGCAAAISAQPNDPVEYL GLWLLKYVKNAEVEGNFYRE RQQDLQKKKDRLVKEAQSEQ AAKSVALTRKEAADALALVT AEPRELLEAAVKLVKQHTAA GAAYAAVVAEPEEPDWVAPE DDEAAAVETEDEAAGGAALA EGEEPPPEPEPEPEAAPEDG EGDAPAPKIPRPVDYSKKYF AYVAASAGQEHVLEADLYRP APPPEDADEDFKPEPLPYSF RVLDEKLPMLYVPNVAAEER VKFFRKFPKIGSYQACGVAL PASGEFKALLAADTLFPEGS GQPLSADDRDFVWEVSQSLS RALEAVQARAAEALAATSAA EAVEELKAKVAELREQAAAE AAAAAPPPPAEGEEGEGEAP PAEEEPPAEEEAEEEEEEAE EGAEEGAEEGEEGEEAPPKP KKKKKVFNPIPGLQAAIEKL TAAAEAATEADARAQAAVAL EKQALDEVVALASSHSDATL SSLRNMLSVPQGTYHVVKAL LHLLGRPAASFSTWKRAHSH FSPRLFEDMAAYDATAERDM AVWGRVRSCYKAAPAAKKLD AEMPNTLFGSVALMYIKQVR RVARKAVLQRELAAKLAKAQ QDLADKQAALVEAERVKAER EAEEARLAAEAEAAAAAEAE AAARAAAEAEAAAAAEAAAE AAAEAAAAAAEAAAEAGEGE AVAEREAAPAEAEAAPAEGE AAPPAEGEGEAQPAQEGSNS SSSSSDSSSSEESKAAAE

Data sets:
Data typeCount
kinetic rates1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RSP21
2ATP2

Entities:

Entity 1, RSP2 738 residues - 507.181 Da.

1   METALAPROTHRGLNALAGLYHISASPTHR
2   ALATYRLEULYSGLUTHRVALGLYGLUALA
3   LEUALAARGGLYCYSALAALAALAILESER
4   ALAGLNPROASNASPPROVALGLUTYRLEU
5   GLYLEUTRPLEULEULYSTYRVALLYSASN
6   ALAGLUVALGLUGLYASNPHETYRARGGLU
7   ARGGLNGLNASPLEUGLNLYSLYSLYSASP
8   ARGLEUVALLYSGLUALAGLNSERGLUGLN
9   ALAALALYSSERVALALALEUTHRARGLYS
10   GLUALAALAASPALALEUALALEUVALTHR
11   ALAGLUPROARGGLULEULEUGLUALAALA
12   VALLYSLEUVALLYSGLNHISTHRALAALA
13   GLYALAALATYRALAALAVALVALALAGLU
14   PROGLUGLUPROASPTRPVALALAPROGLU
15   ASPASPGLUALAALAALAVALGLUTHRGLU
16   ASPGLUALAALAGLYGLYALAALALEUALA
17   GLUGLYGLUGLUPROPROPROGLUPROGLU
18   PROGLUPROGLUALAALAPROGLUASPGLY
19   GLUGLYASPALAPROALAPROLYSILEPRO
20   ARGPROVALASPTYRSERLYSLYSTYRPHE
21   ALATYRVALALAALASERALAGLYGLNGLU
22   HISVALLEUGLUALAASPLEUTYRARGPRO
23   ALAPROPROPROGLUASPALAASPGLUASP
24   PHELYSPROGLUPROLEUPROTYRSERPHE
25   ARGVALLEUASPGLULYSLEUPROMETLEU
26   TYRVALPROASNVALALAALAGLUGLUARG
27   VALLYSPHEPHEARGLYSPHEPROLYSILE
28   GLYSERTYRGLNALACYSGLYVALALALEU
29   PROALASERGLYGLUPHELYSALALEULEU
30   ALAALAASPTHRLEUPHEPROGLUGLYSER
31   GLYGLNPROLEUSERALAASPASPARGASP
32   PHEVALTRPGLUVALSERGLNSERLEUSER
33   ARGALALEUGLUALAVALGLNALAARGALA
34   ALAGLUALALEUALAALATHRSERALAALA
35   GLUALAVALGLUGLULEULYSALALYSVAL
36   ALAGLULEUARGGLUGLNALAALAALAGLU
37   ALAALAALAALAALAPROPROPROPROALA
38   GLUGLYGLUGLUGLYGLUGLYGLUALAPRO
39   PROALAGLUGLUGLUPROPROALAGLUGLU
40   GLUALAGLUGLUGLUGLUGLUGLUALAGLU
41   GLUGLYALAGLUGLUGLYALAGLUGLUGLY
42   GLUGLUGLYGLUGLUALAPROPROLYSPRO
43   LYSLYSLYSLYSLYSVALPHEASNPROILE
44   PROGLYLEUGLNALAALAILEGLULYSLEU
45   THRALAALAALAGLUALAALATHRGLUALA
46   ASPALAARGALAGLNALAALAVALALALEU
47   GLULYSGLNALALEUASPGLUVALVALALA
48   LEUALASERSERHISSERASPALATHRLEU
49   SERSERLEUARGASNMETLEUSERVALPRO
50   GLNGLYTHRTYRHISVALVALLYSALALEU
51   LEUHISLEULEUGLYARGPROALAALASER
52   PHESERTHRTRPLYSARGALAHISSERHIS
53   PHESERPROARGLEUPHEGLUASPMETALA
54   ALATYRASPALATHRALAGLUARGASPMET
55   ALAVALTRPGLYARGVALARGSERCYSTYR
56   LYSALAALAPROALAALALYSLYSLEUASP
57   ALAGLUMETPROASNTHRLEUPHEGLYSER
58   VALALALEUMETTYRILELYSGLNVALARG
59   ARGVALALAARGLYSALAVALLEUGLNARG
60   GLULEUALAALALYSLEUALALYSALAGLN
61   GLNASPLEUALAASPLYSGLNALAALALEU
62   VALGLUALAGLUARGVALLYSALAGLUARG
63   GLUALAGLUGLUALAARGLEUALAALAGLU
64   ALAGLUALAALAALAALAALAGLUALAGLU
65   ALAALAALAARGALAALAALAGLUALAGLU
66   ALAALAALAALAALAGLUALAALAALAGLU
67   ALAALAALAGLUALAALAALAALAALAALA
68   GLUALAALAALAGLUALAGLYGLUGLYGLU
69   ALAVALALAGLUARGGLUALAALAPROALA
70   GLUALAGLUALAALAPROALAGLUGLYGLU
71   ALAALAPROPROALAGLUGLYGLUGLYGLU
72   ALAGLNPROALAGLNGLUGLYSERASNSER
73   SERSERSERSERSERASPSERSERSERSER
74   GLUGLUSERLYSALAALAALAGLU

Entity 2, ATP - C10 H16 N5 O13 P3 - 507.181 Da.

1   ATP

Samples:

sample_1: sodium chloride 200 mM; sodium phosphate 20 mM; RSP2 10 uM; ATP 1 mM; GTP 1 mM; CMP 1 mM; PCG 1 mM; cCMP 1 mM; AMP 1 mM; D2O 100%

sample_2: sodium chloride 200 mM; sodium phosphate 20 mM; RSP2 10 uM; ATP 1 mM; GTP 1 mM; CMP 1 mM; PCG 1 mM; cCMP 1 mM; AMP 1 mM; MG 4 mM; D2O 100%

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
1D STD 1H NMRsample_1isotropicsample_conditions_1
1D STD 1H NMRsample_2isotropicsample_conditions_1

Software:

MestRe-C, (University of Santiago, Spain) - processing

SigmaPlot v8.0, aspire software international - data analysis

NMR spectrometers:

  • Varian 300 121.5 MHz

Related Database Links:

GB AAQ92371 EDP06497
REF XP_001702718
SP Q6UBQ3
AlphaFold Q6UBQ3