BMRB Entry 17093

Title:
Structure and dynamics of a molten globular enzyme
Deposition date:
2010-08-02
Original release date:
2010-11-10
Authors:
Pervushin, Konstantin; Vamvaca, Katherina; Vogeli, Beat; Hilvert, Donald
Citation:

Citation: Pervushin, Konstantin; Vamvaca, Katherina; Vogeli, Beat; Hilvert, Donald. "Structure and dynamics of a molten globular enzyme"  Nat. Struct. Biol. 14, 1202-1206 (2007).
PubMed: 17994104

Assembly members:

Assembly members:
mMjCM, polymer, 104 residues, Formula weight is not available
8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC ACID, non-polymer, 228.199 Da.

Natural source:

Natural source:   Common Name: Methanococcus jannaschii   Taxonomy ID: 2190   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanococcus jannaschii

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Escherichia coli   Vector: na

Data sets:
Data typeCount
binding constants2
kinetic rates4

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mMjCM1
2TSA2

Entities:

Entity 1, mMjCM 104 residues - Formula weight is not available

1   METILEGLULYSLEUALAGLUILEARGLYS
2   LYSILEASPGLUILEASPASNLYSILELEU
3   LYSALAARGTRPPROTRPALAGLULYSLEU
4   ILEALAGLUARGASNSERLEUALALYSASP
5   VALALAGLUILELYSASNGLNLEUGLYILE
6   PROILEASNASPPROGLUARGGLULYSTYR
7   ILETYRASPARGILEARGLYSLEUCYSLYS
8   GLUHISASNVALASPGLUASNILEGLYILE
9   LYSILEPHEGLNARGLEUILEGLUHISASN
10   LYSALALEUGLNLYSGLNTYRLEUGLUGLU
11   THRLEUGLUHIS

Entity 2, TSA - C10 H12 O6 - 228.199 Da.

1   TSA

Samples:

sample_1: sodium phosphate 20 mM; sodium chloride 50 mM; mMjCM, [U-13C; U-15N], 0.6 mM; D2O 5%; H2O 95%; TSA 2.5 mM

sample_2: sodium phosphate 20 mM; sodium chloride 50 mM; mMjCM, [U-13C; U-15N], 0.1 uM; D2O 5%; H2O 95%; TSA 64 uM

sample_3: sodium phosphate 20 mM; sodium chloride 50 mM; mMjCM, [U-13C; U-15N], 0.1 uM; D2O 5%; H2O 95%; TSA 90 uM

sample_4: sodium phosphate 20 mM; sodium chloride 50 mM; mMjCM, [U-13C; U-15N], 0.1 uM; D2O 5%; H2O 95%; TSA 300 uM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_4isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 900 MHz

Related Database Links:

PDB