BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17092

Title: NMR solution structure of the N-terminal domain of DNA-binding protein SATB1 from Homo sapiens: Northeast Structural Genomics Target HR4435B(179-250)

Deposition date: 2010-08-02 Original release date: 2012-08-02

Authors: Swapna, G. V. T.; ., Montelione; ., .; ., .; ., .; 7, .; John, 8; Montelione, Gaetano

Citation: Swapna, G. V. T.; ., Montelione; ., .; ., .; ., .; 17092, .; 8, 17092; Gaetano, 9. "NMR solution structure of the N-terminal domain of DNA-binding protein SATB1 from Homo sapiens: Northeast Structural Genomics Target HR4435B(179-250)"  .

Assembly members:
entity, polymer, 83 residues, 9699.189 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology' 'Escherichia coli' . . . Escherichia coli 'BL21(DE3)+ Magic' 'pET 14-15C

Entity Sequences (FASTA):
entity: MGHHHHHHSHMLPPEQWSHT TVRNALKDLLKDMNQSSLAK ECPLSQSMISSIVNSTYYAN VSAAKCQEFGRWYKHFKKTK DMM

Data sets:
Data typeCount
13C chemical shifts326
791H chemical shifts

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SATB11

Entities:

Entity 1, SATB1 83 residues - 9699.189 Da.

Consists of an N-terminal tag MGHHHHHHSH.

1   MET.170925.HIS.1709211.
2   PRO.1709217.HIS.1709223.
3   ALA.1709229.LYS.1709235.
4   SER.1709241.PRO.1709247.
5   ILE.1709253.SER.1709259.
6   VAL.1709265.GLN.1709271.
7   TYR.1709277.THR.1709283

Samples:

HR4435B.009: HR4435B, [U-100% 13C; U-100% 15N], 1.08 ± 0.2 mM; natural abundance; .; . %; .; . 17092; . 8; ., 90, 17092 – 9 D2O; 10 17092

HR4435B.009_conditions: ionic strength: 105 mM; 6.5: . 17092; .: atm temperature; K: 17092

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCHR4435B.009isotropicHR4435B.009_conditions
nosotropicnot availablenot available
not availablenot availablenot availablenot available
not availablenot available1not available
not availablenot available$HR4435B.009_conditionsnot available
not availableHR4435B.009_conditionsnot availablespectrometer_1
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot available2not available
not availablenot available$spectrometer_2not available
not availablespectrometer_2not availablenot available

Software:

AutoStruct v2.2.1, Huang, Tejero, Powers and Montelione - chemical shift assignment, 17092

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - geometry optimization, 17092

CNS v2.0.6, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization, 17092

AutoAssign v2.2.1, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
4Q2J .
. unnamed protein product [Mus musculus] . . . . 86.75 . . . 17092
special AT-rich sequence binding protein 1 variant [Homo sapiens] .
86.75 763
763 100.00
100.00 100.00 1.62e-42
1.62e-42 .
17092 20
21 no

Download simulated HSQC data in one of the following formats:
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