BMRB Entry 17079

Title:
Solution NMR structure ov the chromobox protein Cbx7 with H3K27me3   PubMed: 21047797
Deposition date:
2010-07-27
Original release date:
2010-08-18
Authors:
Kaustov, Lilia; Lemak, Alexander; Fares, Christophe; Gutmanas, Aleksandras; Muhandiram, Ranjith; Quang, Hui; Loppnau, Peter; Min, Jinrong; Edwards, Al; Arrowsmith, Cheryl
Citation:

Citation: Kaustov, Lilia; Ouyang, Hui; Amaya, Maria; Lemak, Alexander; Nady, Nataliya; Duan, Shili; Wasney, Gregory; Li, Zhihong; Vedadi, Masoud; Schapira, Matthieu; Min, Jinrong; Arrowsmith, Cheryl. "Recognition and specificity determinants of the human cbx chromodomains."  J. Biol. Chem. 286, 521-529 (2011).

Assembly members:

Assembly members:
Cbx7, polymer, 56 residues, 6816.929 Da.
H3K27me3, polymer, 15 residues, 1515.792 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a-mhl

Experimental source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a-mhl

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts254
15N chemical shifts50
1H chemical shifts473

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cbx71
2H3K27me32

Entities:

Entity 1, Cbx7 56 residues - 6816.929 Da.

1   GLYGLUGLNVALPHEALAVALGLUSERILE
2   ARGLYSLYSARGVALARGLYSGLYLYSVAL
3   GLUTYRLEUVALLYSTRPLYSGLYTRPPRO
4   PROLYSTYRSERTHRTRPGLUPROGLUGLU
5   HISILELEUASPPROARGLEUVALMETALA
6   TYRGLUGLULYSGLUGLU

Entity 2, H3K27me3 15 residues - 1515.792 Da.

1   GLNLEUALATHRLYSALAALAARGM3LSER
2   ALAPROALATHRGLY

Samples:

sample_1: Cbx7, [U-13C; U-15N], 0.5 mM; H3K27me3 2.7 mM; sodium phosphate 10 mM; sodium chloride 300 mM; PMSF 0.5 mM; TCEP 0.5 mM; Benzamidine 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 300 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
13C/15N-filtered/edited NOESYsample_1isotropicsample_conditions_1
3D aro 1H-13C NOESYsample_1isotropicsample_conditions_1
3D aro HCCH-TOCSYsample_1isotropicsample_conditions_1
3D aro (H)CCH-TOCSYsample_1isotropicsample_conditions_1

Software:

ABACUS, (ABACUS)-Grishaev - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - peak picking

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Varian INOVA 500 MHz

Related Database Links:

BMRB 15674 16778 17072
PDB
DBJ BAI46775
GB AAH21398 AAH51773 AAH62392 AAR26721 AAX94038
REF NP_001179547 NP_001247702 NP_659060 NP_783640 NP_954548
SP O95931 P60889 Q8VDS3
TPG DAA29144
AlphaFold O95931

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts