BMRB Entry 17078

Name: 1H, 15N chemical shift assignments of the imino groups in the base pairs of Escherichia coli tRNALeu (CAG)
Published: 2010-10-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17078

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 15N chemical shift assignments of the imino groups in the base pairs of Escherichia coli tRNALeu (CAG)

Deposition date:

2010-07-27

Original release date:

2010-10-14

Authors:

Hao, Zhan-Xi; Feng, Rui; Wang, En-Duo; Zhu, Guang

Citation:

Citation: Hao, Zhan-Xi; Feng, Rui; Wang, En-Duo; Zhu, Guang. "(1)H, (15)N chemical shift assignments of the imino groups in the base pairs of Escherichia coli tRNA(Leu) (CAG)." Biomol. NMR Assignments 5, 71-74 (2011).
PubMed: 20931304

Assembly members:

Assembly members:
leucine_transfer_ribonucleic_acid, polymer, 87 residues, Formula weight is not available
MG, non-polymer, 24.305 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTrc99B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
leucine_transfer_ribonucleic_acid: GCGAAGGUGGCGGAAXXXGX AGACGCGCUAGCUUCAGXXG XUAGUGUCCUUACGGACGUG GGGGXXCAAGUCCCCCCCCU CGCACCA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	32
1H chemical shifts	32

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	tRNA monomer	1
2	magnesium ion	2

Entities:

Entity 1, tRNA monomer 87 residues - Formula weight is not available

1

G

C

G

A

G

U

G

2

C

G

A

H2U

OMG

G

H2U

3

A

G

A

C

G

C

G

C

U

A

4

G

C

U

C

A

G

1MG

PSU

G

5

PSU

U

A

G

U

G

U

C

U

6

U

A

C

G

A

C

G

U

G

7

G

5MU

PSU

C

A

G

8

U

C

U

9

C

G

C

A

C

A

Entity 2, magnesium ion - Mg - 24.305 Da.

1

MG

Samples:

15N-EctRNALeu(CAG): tRNALeu, [U-95% 15N], 1 mM; MgCl2 10 mM; NaCl 100 mM; sodium phosphate 10 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	15N-EctRNALeu(CAG)	isotropic	sample_conditions_1
2D 1H-1H NOESY	15N-EctRNALeu(CAG)	isotropic	sample_conditions_1
3D 1H-15N NOESY	15N-EctRNALeu(CAG)	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 750 MHz