BMRB Entry 17057
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17057
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NMR-STAR v3 text file.
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Citation: Sriram, Renuka; Kreutzer, Ulrike; Shih, Lifan; Jue, Thomas. "Interaction of fatty acid with myoglobin" FEBS Lett. 582, 3643-3649 (2008).
PubMed: 18840435
Assembly members:
Mb, polymer, 154 residues, Formula weight is not available
PALMITIC ACID, non-polymer, 256.424 Da.
CYANIDE ION, non-polymer, 26.017 Da.
Natural source: Common Name: Horse Taxonomy ID: 9796 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Equus caballus
Experimental source: Production method: obtained from a vendor
- binding_constants
| Data type | Count |
| binding constants | 2 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Mb | 1 |
| 2 | PLM | 2 |
| 3 | CYN | 3 |
Entities:
Entity 1, Mb 154 residues - Formula weight is not available
| 1 | MET | GLY | LEU | SER | ASP | GLY | GLU | TRP | GLN | GLN | ||||
| 2 | VAL | LEU | ASN | VAL | TRP | GLY | LYS | VAL | GLU | ALA | ||||
| 3 | ASP | ILE | ALA | GLY | HIS | GLY | GLN | GLU | VAL | LEU | ||||
| 4 | ILE | ARG | LEU | PHE | THR | GLY | HIS | PRO | GLU | THR | ||||
| 5 | LEU | GLU | LYS | PHE | ASP | LYS | PHE | LYS | HIS | LEU | ||||
| 6 | LYS | THR | GLU | ALA | GLU | MET | LYS | ALA | SER | GLU | ||||
| 7 | ASP | LEU | LYS | LYS | HIS | GLY | THR | VAL | VAL | LEU | ||||
| 8 | THR | ALA | LEU | GLY | GLY | ILE | LEU | LYS | LYS | LYS | ||||
| 9 | GLY | HIS | HIS | GLU | ALA | GLU | LEU | LYS | PRO | LEU | ||||
| 10 | ALA | GLN | SER | HIS | ALA | THR | LYS | HIS | LYS | ILE | ||||
| 11 | PRO | ILE | LYS | TYR | LEU | GLU | PHE | ILE | SER | ASP | ||||
| 12 | ALA | ILE | ILE | HIS | VAL | LEU | HIS | SER | LYS | HIS | ||||
| 13 | PRO | GLY | ASP | PHE | GLY | ALA | ASP | ALA | GLN | GLY | ||||
| 14 | ALA | MET | THR | LYS | ALA | LEU | GLU | LEU | PHE | ARG | ||||
| 15 | ASN | ASP | ILE | ALA | ALA | LYS | TYR | LYS | GLU | LEU | ||||
| 16 | GLY | PHE | GLN | GLY |
Entity 2, PLM - C16 H32 O2 - 256.424 Da.
| 1 | PLM |
Entity 3, CYN - C N - 26.017 Da.
| 1 | CYN |
Samples:
sample_1: PLM0 – 0.4 mM; Mb 0.8 mM; Tris buffer 30 mM; EDTA 1 mM; CN 0.8 mM; H2O 95%; D2O 5%
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H NMR | sample_1 | isotropic | sample_conditions_1 |
Software:
SigmaPlot, Systat Software, Inc., Point Richmond, CA - statistical analysis
NMR spectrometers:
- Bruker Avance 500 MHz