BMRB Entry 17048

Name: AP180 aa 623-680 Backbone Assignment
Published: 2012-08-02

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17048

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

AP180 aa 623-680 Backbone Assignment

Deposition date:

2010-07-06

Original release date:

2012-08-02

Authors:

Zhuo, Yue; Ilangovan, Udayar; Hinck, Andrew; Lafer, Eileen

Citation:

Citation: Zhuo, Yue; Ilangovan, Udayar; Schirf, Virgil; Demeler, Borries; Sousa, Rui; Hinck, Andrew; Lafer, Eileen. "Dynamic interactions between clathrin and locally structured elements in a disordered protein mediate clathrin lattice assembly." J. Mol. Biol. 404, 274-290 (2010).
PubMed: 20875424

Assembly members:

Assembly members:
AP180_M5, polymer, 58 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AP180_M5: ASTASPAKAESSGVIDLFGD AFGSGASETQPAPQAVSSSS ASADLLAGFGGSFMAPST

Data sets:

assigned_chemical_shifts
- AP180_M5_Backbone_Assigned_Chem_Shift_List

Data type	Count
13C chemical shifts	151
15N chemical shifts	45
1H chemical shifts	92

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AP180 M5	1

Entities:

Entity 1, AP180 M5 58 residues - Formula weight is not available

1

ALA

SER

THR

ALA

SER

PRO

ALA

LYS

ALA

GLU

2

SER

GLY

VAL

ILE

ASP

LEU

PHE

GLY

ASP

3

ALA

PHE

GLY

SER

GLY

ALA

SER

GLU

THR

GLN

4

PRO

ALA

PRO

GLN

ALA

VAL

SER

5

ALA

SER

ALA

ASP

LEU

ALA

GLY

PHE

GLY

6

GLY

SER

PHE

MET

ALA

PRO

SER

THR

Samples:

sample_1: AP180 M5, [U-13C; U-15N], 300 – 500 uM; disodium phosphate 25 mM; sodium chloride 50 mM; sodium azide 0.2%; H2O 95%; D2O 5%

AP180_M5_Sample_Conditions: ionic strength: 125 mM; pH: 7.0; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	AP180_M5_Sample_Conditions
3D HNCA	sample_1	isotropic	AP180_M5_Sample_Conditions
3D HNCACB	sample_1	isotropic	AP180_M5_Sample_Conditions
3D CBCA(CO)NH	sample_1	isotropic	AP180_M5_Sample_Conditions
3D HNCO	sample_1	isotropic	AP180_M5_Sample_Conditions

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

Bruker Avance 500 MHz
Bruker DRX 600 MHz

DBJ	BAC41439
EMBL	CAA48748 CAA48749
GB	AAA37586 AAA37587 AAH31773 AAH52454 EDL26508
REF	NP_001264911 NP_001264915 NP_038697 NP_113916 XP_006243553
SP	Q05140 Q61548
AlphaFold	Q05140 Q61548