BMRB Entry 17042

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17042

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone 1H, 15N, and 13C resonance assignment of rat-nNOS fragment (residues 134-298)
Deposition date:
2010-07-02
Original release date:
2010-07-13
Authors:
Kumar, Dinesh; Hosur, Ramakrishna; Parhad, Swapnil; Ray, Krishanu; Mazumdar, Shyamalava
Citation:

Citation: Parhad, Swapnil; Kumar, Dinesh; Hosur, Ramakrishna; Ray, Krishanu; Mazumdar, Shyamalava. "Backbone 1H, 15N, and 13C resonance assignment of rat nNOS fragment (residues 134-298)"  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
nNOS134-298, polymer, 170 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
nNOS134-298: GSPEFDLSHQPSASKDQSLA VDRVTGLGNGPQHAQGHGQG AGSVSQANGVAIDPTMKSTK ANLQDIGEHDELLKEIEPVL SILNSGSKATNRGGPAKAEM KDTGIQVDRDLDGKSHKAPP LGGDNDRVFNDLWGKDNVPV VLNNPYSEKEQSPTSGKQSP TKNGSPSRCP

Data sets:
Data typeCount
13C chemical shifts315
15N chemical shifts119
1H chemical shifts119

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nNOS fragment1

Entities:

Entity 1, nNOS fragment 170 residues - Formula weight is not available

1   GLYSERPROGLUPHEASPLEUSERHISGLN
2   PROSERALASERLYSASPGLNSERLEUALA
3   VALASPARGVALTHRGLYLEUGLYASNGLY
4   PROGLNHISALAGLNGLYHISGLYGLNGLY
5   ALAGLYSERVALSERGLNALAASNGLYVAL
6   ALAILEASPPROTHRMETLYSSERTHRLYS
7   ALAASNLEUGLNASPILEGLYGLUHISASP
8   GLULEULEULYSGLUILEGLUPROVALLEU
9   SERILELEUASNSERGLYSERLYSALATHR
10   ASNARGGLYGLYPROALALYSALAGLUMET
11   LYSASPTHRGLYILEGLNVALASPARGASP
12   LEUASPGLYLYSSERHISLYSALAPROPRO
13   LEUGLYGLYASPASNASPARGVALPHEASN
14   ASPLEUTRPGLYLYSASPASNVALPROVAL
15   VALLEUASNASNPROTYRSERGLULYSGLU
16   GLNSERPROTHRSERGLYLYSGLNSERPRO
17   THRLYSASNGLYSERPROSERARGCYSPRO

Samples:

sample_1: nNOS134-298, [U-99% 13C; U-99% 15N], 800 ± 100 uM; D2O 90%; DTT 1 mM; H2O 10%; potassium phosphate 100 mM; potassium chloride 300 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
HNNsample_1isotropicsample_conditions_1
hncoCANHsample_1isotropicsample_conditions_1
hnCOcaNHsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

EMBL CAA42574
GB AAC52782
PRF 1712320A
REF NP_434686 XP_008767432
SP P29476
AlphaFold P29476

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks