BMRB Entry 17036

Name: SOLUTION STRUCTURE OF ZINC FINGER DOMAIN OF E3 UBIQUITIN-PROTEIN LIGASE PROTEIN Praja-1 FROM HOMO SAPIENS, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET HR4710B
Published: 2010-08-24

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17036

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SOLUTION STRUCTURE OF ZINC FINGER DOMAIN OF E3 UBIQUITIN-PROTEIN LIGASE PROTEIN Praja-1 FROM HOMO SAPIENS, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET HR4710B

Deposition date:

2010-06-30

Original release date:

2010-08-24

Authors:

Liu, Gaohua; Tong, Sai; Hamilton, Keith; Ciccosanti, Colleen; Shastry, Ritu; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano

Citation:

Citation: Liu, Gaohua; Tong, Sai; Xiao, Rong; Acton, Thomas; Everett, John; Montelione, Gaetano. "SOLUTION STRUCTURE OF ZINC FINGER DOMAIN OF E3 UBIQUITIN-PROTEIN LIGASE PROTEIN Praja-1 FROM HOMO SAPIENS, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET HR4710B" .

Assembly members:

Assembly members:
pja1_zinc_finger_domain, polymer, 91 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 14-15C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
pja1_zinc_finger_domain: MGHHHHHHSHMVANPPASKE SIDALPEILVTEDHGAVGQE MCCPICCSEYVKGDVATELP CHHYFHKPCVSIWLQKSGTC PVCRCMFPPPL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
spectral_peak_list

Data type	Count
13C chemical shifts	277
15N chemical shifts	73
1H chemical shifts	554

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	pja1 zinc finger domain	1

Entities:

Entity 1, pja1 zinc finger domain 91 residues - Formula weight is not available

1

MET

GLY

HIS

SER

HIS

2

MET

VAL

ALA

ASN

PRO

ALA

SER

LYS

GLU

3

SER

ILE

ASP

ALA

LEU

PRO

GLU

ILE

LEU

VAL

4

THR

GLU

ASP

HIS

GLY

ALA

VAL

GLY

GLN

GLU

5

MET

CYS

PRO

ILE

CYS

SER

GLU

TYR

6

VAL

LYS

GLY

ASP

VAL

ALA

THR

GLU

LEU

PRO

7

CYS

HIS

TYR

PHE

HIS

LYS

PRO

CYS

VAL

8

SER

ILE

TRP

LEU

GLN

LYS

SER

GLY

THR

CYS

9

PRO

VAL

CYS

ARG

CYS

MET

PHE

PRO

10

LEU

Samples:

sample_NC: pja1 zinc finger domain, [U-100% 13C; U-100% 15N], 0.94 mM; H2O 90%; D2O 10%

sample_nc5: pja1 zinc finger domain, [U-10% 13C; U-100% 15N], 0.82 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_NC	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_nc5	isotropic	sample_conditions_1
3D 1H-13C-15N SIMUTANEOUS NOESY	sample_NC	isotropic	sample_conditions_1
3D 1H-13C aromatic NOESY	sample_NC	isotropic	sample_conditions_1
3D HNCACB	sample_NC	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_NC	isotropic	sample_conditions_1
3D C(CO)NH	sample_NC	isotropic	sample_conditions_1
3D HNCO	sample_NC	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_NC	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_NC	isotropic	sample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization, refinement, structure solution

CYANA, Guntert, Mumenthaler and Wuthrich - geometry optimization, refinement, structure solution

AutoStruct, Huang, Tejero, Powers and Montelione - data analysis, geometry optimization, refinement

TALOS+, Shen, Delaglio and Bax - data analysis

XEASY, Bartels et al. - data analysis, peak picking, refinement

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 800 MHz
Varian INOVA 600 MHz

PDB	2L0B
DBJ	BAB13928 BAB23982 BAE22512 BAE73073 BAE88506
EMBL	CAH93231
GB	AAC00205 AAH08148 AAH25975 AAH37616 AAH48323
REF	NP_001027568 NP_001076579 NP_001094476 NP_001128898 NP_001277484
SP	O55176 Q8NG27
AlphaFold	O55176 Q8NG27