BMRB Entry 17035

Name: NMR Solution Structure of asr4154 from Nostoc sp. PCC7120 Northeast Structural Genomics Consortium target ID Nsr143
Published: 2011-12-15

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PDB ID: 2l09
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17035
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Solution Structure of asr4154 from Nostoc sp. PCC7120 Northeast Structural Genomics Consortium target ID Nsr143

Deposition date:

2010-06-30

Original release date:

2011-12-15

Authors:

Feldmann, Erik; Yang, Yunhuang; Ramelot, Theresa; Cort, John; Janjua, Haleema; Ciccosanti, Colleen; Lee, Dan; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano; Kennedy, Michael

Citation:

Citation: Feldmann, Erik; Yang, Yunhuang; Ramelot, Theresa; Cort, John; Janjua, Haleema; Ciccosanti, Colleen; Lee, Dan; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano; Kennedy, Michael. "NMR Solution Structure of asr4154 from Nostoc sp. PCC7120 Northeast Structural Genomics target ID Nsr143" .

Assembly members:

Assembly members:
Hypothetical_protein_asr4154_from_Nostoc_sp._PCC7120, polymer, 62 residues, 7352 Da.

Natural source:

Natural source: Common Name: Nostoc sp. PCC 7120 Taxonomy ID: 103690 Superkingdom: Bacteria Kingdom: Nostoc Genus/species: not available Nostoc sp. PCC 7120

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 21-23C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hypothetical_protein_asr4154_from_Nostoc_sp._PCC7120: MNLRWTSEAKTKLKNIPFFA RSQAKARIEQLARQAEQDIV TPELVEQARLEFGQLEHHHH HH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
spectral_peak_list

Data type	Count
13C chemical shifts	254
15N chemical shifts	67
1H chemical shifts	414

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	asr4154	1

Entities:

Entity 1, asr4154 62 residues - 7352 Da.

Native asr4154 protein followed by 8 non-native C-terminal residues (LEHHHHHH)

1

MET

ASN

LEU

ARG

TRP

THR

SER

GLU

ALA

LYS

2

THR

LYS

LEU

LYS

ASN

ILE

PRO

PHE

ALA

3

ARG

SER

GLN

ALA

LYS

ALA

ARG

ILE

GLU

GLN

4

LEU

ALA

ARG

GLN

ALA

GLU

GLN

ASP

ILE

VAL

5

THR

PRO

GLU

LEU

VAL

GLU

GLN

ALA

ARG

LEU

6

GLU

PHE

GLY

GLN

LEU

GLU

HIS

7

HIS

Samples:

NC_sample: Hypothetical protein asr4154 from Nostoc sp. PCC7120, [U-100% 13C; U-100% 15N], 0.9 ± 0.09 mM; MES 20 ± 1 mM; sodium chloride 100 ± 5 mM; calcium chloride 5 ± 0.2 mM; DTT 10 ± 0.5 mM; sodium azide 0.02 ± 0.001 %; H2O 90%; D2O 10%

NC5_sample: Hypothetical protein asr4154 from Nostoc sp. PCC7120, [U-5% 13C; U-100% 15N], 0.82 ± 0.08 mM; MES 20 ± 1 mM; sodium chloride 100 ± 5 mM; calcium chloride 5 ± 0.2 mM; DTT 10 ± 0.5 mM; sodium azide 0.02 ± 0.001 %; H2O 90%; D2O 10%

NC_sample_in_D2O: Hypothetical protein asr4154 from Nostoc sp. PCC7120, [U-100% 13C; U-100% 15N], 0.9 ± 0.09 mM; MES 20 ± 1 mM; sodium chloride 100 ± 5 mM; calcium chloride 5 ± 0.2 mM; DTT 10 ± 0.5 mM; sodium azide 0.02 ± 0.001 %; D2O 100%

sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	NC_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC_CT	NC5_sample	isotropic	sample_conditions_1
3D 1H-15N NOESY	NC_sample	isotropic	sample_conditions_1
3D 1H-13C NOESY	NC_sample	isotropic	sample_conditions_1
3D HNCO	NC_sample	isotropic	sample_conditions_1
3D HNCA	NC_sample	isotropic	sample_conditions_1
3D HNCACB	NC_sample	isotropic	sample_conditions_1
3D CBCA(CO)NH	NC_sample	isotropic	sample_conditions_1
3D HN(CO)CA	NC_sample	isotropic	sample_conditions_1
3D HBHA(CO)NH	NC_sample	isotropic	sample_conditions_1
3D H(CCO)NH	NC_sample	isotropic	sample_conditions_1
3D C(CO)NH	NC_sample	isotropic	sample_conditions_1
3D HCCH-TOCSY	NC_sample	isotropic	sample_conditions_1
3D HCCH-COSY	NC_sample	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	NC_sample_in_D2O	isotropic	sample_conditions_1
4D CC NOESY	NC_sample_in_D2O	isotropic	sample_conditions_1
2D 1H-15N HSQC_swN150ppm	NC_sample	isotropic	sample_conditions_1

Software:

NMRPipe v2008, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMR v6.1C, Varian - collection

TOPSPIN v2.1.4, Bruker Biospin - collection

AutoStruct v2.2.1, Huang, Tejero, Powers and Montelione - data analysis

X-PLOR NIH v2.20, Schwieters, Kuszewski, Tjandra and Clore - structure solution

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

SPARKY v3.113, Goddard - data analysis

PSVS v1.4, Bhattacharya and Montelione - refinement

AutoAssign v2.30, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

PDBStat v5.1, (PdbStat)-Roberto Tejero and Gaetano T. Montelione - structure solution

PINE v1.0, Bahrami, A., Assadi, A., Markley, J. L. & Eghbalnia, H. - autoassignment

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Varian INOVA 600 MHz
Bruker AvanceIII 850 MHz
Varian INOVA 500 MHz

PDB	2L09
DBJ	BAB75853
GB	ABA20375
REF	WP_010998293