BMRB Entry 17029

Name: 1H, 15N and 13C assignments for the potential GTPase binding domain of dictyostelium discoideum Fomin C
Published: 2011-10-28

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17029

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C assignments for the potential GTPase binding domain of dictyostelium discoideum Fomin C

Deposition date:

2010-06-30

Original release date:

2011-10-28

Authors:

Dames, Sonja; Schoenichen, Andre; Stephan, Grzesiek; Geyer, Matthias

Citation:

Citation: Dames, Sonja; Junemann, Alexander; Sass, Hans; Schonichen, Andre; Stopschinski, Barbara; Grzesiek, Stephan; Faix, Jan; Geyer, Matthias. "Structure, Dynamics, Lipid Binding, and Physiological Relevance of the Putative GTPase-binding Domain of Dictyostelium Formin C." J. Biol. Chem. 286, 36907-36920 (2011).
PubMed: 21846933

Assembly members:

Assembly members:
formin_C, polymer, 107 residues, 12057.7 Da.

Natural source:

Natural source: Common Name: Dictyostelium discoideum Taxonomy ID: 44689 Superkingdom: Eukaryota Kingdom: not available Genus/species: Dictyostelium discoideum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T1 tev

Entity Sequences (FASTA):

Entity Sequences (FASTA):
formin_C: GAHMGGSMKIRVELINGNEH RTSSTPQQPQQNPSVSHIFD GETAVKDHIKVLLTHFKIPV DKVSSYALQNPFTLAYVEDS FLTPERLVEAEKSYFILRMK PHAIADR

Data sets:

assigned_chemical_shifts
- assignments_forC_3l

Data type	Count
13C chemical shifts	482
15N chemical shifts	111
1H chemical shifts	782

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	formin C	1

Entities:

Entity 1, formin C 107 residues - 12057.7 Da.

1

GLY

ALA

HIS

MET

GLY

SER

MET

LYS

ILE

2

ARG

VAL

GLU

LEU

ILE

ASN

GLY

ASN

GLU

HIS

3

ARG

THR

SER

THR

PRO

GLN

PRO

GLN

4

GLN

ASN

PRO

SER

VAL

SER

HIS

ILE

PHE

ASP

5

GLY

GLU

THR

ALA

VAL

LYS

ASP

HIS

ILE

LYS

6

VAL

LEU

THR

HIS

PHE

LYS

ILE

PRO

VAL

7

ASP

LYS

VAL

SER

TYR

ALA

LEU

GLN

ASN

8

PRO

PHE

THR

LEU

ALA

TYR

VAL

GLU

ASP

SER

9

PHE

LEU

THR

PRO

GLU

ARG

LEU

VAL

GLU

ALA

10

GLU

LYS

SER

TYR

PHE

ILE

LEU

ARG

MET

LYS

11

PRO

HIS

ALA

ILE

ALA

ASP

ARG

Samples:

sample_1: forC/ A, [U-100% 15N], 1.0 mM; potassium phosphate 50 mM; sodium chloride 50 mM; sodium azide 0.2%

sample_2: forC/ A, [U-100% 13C; U-100% 15N], 0.8 mM; potassium phosphate 50 mM; sodium chloride 50 mM; sodium azide 0.2%

sample_3: forC/ A, [U-100% 13C; U-100% 15N], 0.8 mM; potassium phosphate 50 mM; sodium chloride 50 mM; sodium azide 0.2%

sample_4: forC/ A, [U-100% 13C; U-100% 15N], 0.8 mM; potassium phosphate 50 mM; sodium chloride 50 mM; sodium azide 0.2 % v/v; C12E5 8.6 % w/v; hexanol 2 % v/v

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D CCONH-TOCSY	sample_2	isotropic	sample_conditions_1
3D CBCANH	sample_2	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HNHB	sample_1	isotropic	sample_conditions_1
3D HACAHB-COSY	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
2D 13C-{13CO}-1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 13C-{15N}-1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_3	isotropic	sample_conditions_1
2D 1H-15N HSQC-IPAP	sample_4	isotropic	sample_conditions_1
3D HN(CO)CA_Jcaha	sample_4	isotropic	sample_conditions_1
3D HN(CO)CA_Jcacb	sample_4	isotropic	sample_conditions_1
{1H}-15N-NOE	sample_1	isotropic	sample_conditions_1
15N-T1	sample_1	isotropic	sample_conditions_1
15N-T2	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 800 MHz

PDB	2L1A
DBJ	BAC16798
GB	EAL63715
REF	XP_637272
SP	Q54KF1
AlphaFold	Q54KF1