BMRB Entry 16999
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16999
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Lemak, Alexander; Yee, Adelinda; Lee, Hsiau-Wei; Semesi, Anthony; Prestegard, James; Montelione, Gaetano; Arrowsmith, Cheryl. "Solution structure of protein CV0426 from Chromobacterium violaceum." .
Assembly members:
cv0426, polymer, 102 residues, 13259.014 Da.
Natural source: Common Name: Chromobacterium violaceum Taxonomy ID: 536 Superkingdom: Bacteria Kingdom: not available Genus/species: Chromobacterium violaceum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: p15Tv lic
Entity Sequences (FASTA):
cv0426: QGMLLHSVETPRGEILNVSE
QEARDVFGASEQAIADARKA
TILQTLRIERDERLRACDWT
QVQDVVLTADQKATWAKYRQ
ALRDLPETVTDLSQIVWPQL
PV
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 381 |
| 15N chemical shifts | 94 |
| 1H chemical shifts | 642 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cv0426 | 1 |
Entities:
Entity 1, cv0426 102 residues - 13259.014 Da.
| 1 | GLN | GLY | MET | LEU | LEU | HIS | SER | VAL | GLU | THR | ||||
| 2 | PRO | ARG | GLY | GLU | ILE | LEU | ASN | VAL | SER | GLU | ||||
| 3 | GLN | GLU | ALA | ARG | ASP | VAL | PHE | GLY | ALA | SER | ||||
| 4 | GLU | GLN | ALA | ILE | ALA | ASP | ALA | ARG | LYS | ALA | ||||
| 5 | THR | ILE | LEU | GLN | THR | LEU | ARG | ILE | GLU | ARG | ||||
| 6 | ASP | GLU | ARG | LEU | ARG | ALA | CYS | ASP | TRP | THR | ||||
| 7 | GLN | VAL | GLN | ASP | VAL | VAL | LEU | THR | ALA | ASP | ||||
| 8 | GLN | LYS | ALA | THR | TRP | ALA | LYS | TYR | ARG | GLN | ||||
| 9 | ALA | LEU | ARG | ASP | LEU | PRO | GLU | THR | VAL | THR | ||||
| 10 | ASP | LEU | SER | GLN | ILE | VAL | TRP | PRO | GLN | LEU | ||||
| 11 | PRO | VAL |
Samples:
sample_1: cv0426, [U-13C; U-15N], 0.5 mM; MOPS 10 mM; sodium chloride 450 mM; ZnSO4 10 uM; DTT 10 mM; NaN3 0.01%; benzamidine 10 mM; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 450 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C_arom NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC (IPAP) | sample_1 | anisotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - peak picking
FMC, Lemak, Steren, Llinas, Arrowsmith - chemical shift assignment
TALOS, Cornilescu, Delaglio and Bax - data analysis
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks