BMRB Entry 16981

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PDB ID: 2kys
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16981
MolProbity Validation Chart

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Title:
NMR Structure of the SARS Coronavirus Nonstructural Protein Nsp7 in Solution at pH 6.5
Deposition date:
2010-06-07
Original release date:
2010-07-08
Authors:
Johnson, Margaret; Jaudzems, Kristaps; Wuthrich, Kurt
Citation:

Citation: Johnson, Margaret; Jaudzems, Kristaps; Wuthrich, Kurt. "NMR Structure of the SARS-CoV Nonstructural Protein 7 in Solution at pH6.5."  J. Mol. Biol. 402, 619-628 (2010).
PubMed: 20709084

Assembly members:

Assembly members:
nsp7, polymer, 85 residues, 9472.080 Da.

Natural source:

Natural source:   Common Name: SARS coronavirus   Taxonomy ID: 227859   Superkingdom: virus   Kingdom: not available   Genus/species: Betacoronavirus Severe acute respiratory syndrome-related coronavirus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
nsp7: GHSKMSDVKCTSVVLLSVLQ QLRVESSSKLWAQCVQLHND ILLAKDTTEAFEKMVSLLSV LLSMQGAVDINRLCEEMLDN RATLQ

Data sets:
Data typeCount
13C chemical shifts293
15N chemical shifts93
1H chemical shifts630

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1nsp71

Entities:

Entity 1, nsp7 85 residues - 9472.080 Da.

1   GLYHISSERLYSMETSERASPVALLYSCYS
2   THRSERVALVALLEULEUSERVALLEUGLN
3   GLNLEUARGVALGLUSERSERSERLYSLEU
4   TRPALAGLNCYSVALGLNLEUHISASNASP
5   ILELEULEUALALYSASPTHRTHRGLUALA
6   PHEGLULYSMETVALSERLEULEUSERVAL
7   LEULEUSERMETGLNGLYALAVALASPILE
8   ASNARGLEUCYSGLUGLUMETLEUASPASN
9   ARGALATHRLEUGLN

Related Database Links:

PDB 1YSY 2AHM 2KYS
DBJ BAC81346 BAC81347 BAC81360 BAC81361 BAC81374
GB AAP13439 AAP13442 AAP13566 AAP13575 AAP30028
REF NP_828849 NP_828850 NP_828865
SP P0C6F5 P0C6F8 P0C6T7 P0C6U8 P0C6V9
AlphaFold P0C6F5 P0C6F8 P0C6T7 P0C6U8 P0C6V9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks