BMRB Entry 16977

Title:
Resonance assignment of nsp7a from arterivirus   PubMed: 20703834
Deposition date:
2010-06-07
Original release date:
2010-08-19
Authors:
Conte, Maria; Gaudin, Cyril; Manolaridis, Ioannis; Tucker, Paul
Citation:

Citation: Gaudin, Cyril; Manolaridis, Ioannis; Tucker, Paul; Conte, Maria. "Resonance assignment of nsp7 from arterivirus."  Biomol. NMR Assignments 5, 23-25 (2011).

Assembly members:

Assembly members:
nsp7a, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Equine arteritis virus   Taxonomy ID: 11047   Superkingdom: not available   Kingdom: Arteriviridae   Genus/species: not available not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM-11

Experimental source:

Natural source:   Common Name: Equine arteritis virus   Taxonomy ID: 11047   Superkingdom: not available   Kingdom: Arteriviridae   Genus/species: not available not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM-11

Data sets:
Data typeCount
13C chemical shifts506
15N chemical shifts127
1H chemical shifts812

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nsp7a1

Entities:

Entity 1, nsp7a 123 residues - Formula weight is not available

1   GLYLEUTHRALATHRLEUALAALALEUTHR
2   ASPASPASPPHEGLNPHELEUSERASPVAL
3   LEUASPCYSARGALAVALARGSERALAMET
4   ASNLEUARGALAALALEUTHRSERPHEGLN
5   VALALAGLNTYRARGASNILELEUASNALA
6   SERLEUGLNVALASPARGASPALAALAARG
7   SERARGARGLEUMETALALYSLEUALAASP
8   PHEALAVALGLUGLNGLUVALTHRALAGLY
9   ASPARGVALVALVALILEASPGLYLEUASP
10   ARGMETALAHISPHELYSASPASPLEUVAL
11   LEUVALPROLEUTHRTHRLYSVALVALGLY
12   GLYSERARGCYSTHRILECYSASPVALVAL
13   LYSGLUGLU

Samples:

sample_1: nsp7a, [U-100% 13C; U-100% 15N], 0.4 – 0.6 mM; H20 90%; D20 10%; potassium phosphate 20 mM; NaCl 50 nM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMRView, Johnson, One Moon Scientific - data analysis

TOPSPIN, Bruker Biospin - collection

VNMR, Varian - collection

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz
  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAQ56330
EMBL CAA69186 CAA69187 CAC42774 CAC42775
GB AAR14191 AAR14192 AAR14200 AAR14201 ABI64071
REF NP_127506 NP_127507 NP_705589
SP P19811

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts