BMRB Entry 16977

Name: Resonance assignment of nsp7a from arterivirus
Published: 2010-08-19

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PDB ID: 2l8k
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16977
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance assignment of nsp7a from arterivirus

Deposition date:

2010-06-07

Original release date:

2010-08-19

Authors:

Conte, Maria; Gaudin, Cyril; Manolaridis, Ioannis; Tucker, Paul

Citation:

Citation: Gaudin, Cyril; Manolaridis, Ioannis; Tucker, Paul; Conte, Maria. "Resonance assignment of nsp7 from arterivirus." Biomol. NMR Assignments 5, 23-25 (2011).
PubMed: 20703834

Assembly members:

Assembly members:
nsp7a, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Equine arteritis virus Taxonomy ID: 11047 Superkingdom: not available Kingdom: Arteriviridae Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
nsp7a: GLTATLAALTDDDFQFLSDV LDCRAVRSAMNLRAALTSFQ VAQYRNILNASLQVDRDAAR SRRLMAKLADFAVEQEVTAG DRVVVIDGLDRMAHFKDDLV LVPLTTKVVGGSRCTICDVV KEE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	506
15N chemical shifts	127
1H chemical shifts	812

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	nsp7a	1

Entities:

Entity 1, nsp7a 123 residues - Formula weight is not available

1

GLY

LEU

THR

ALA

THR

LEU

ALA

LEU

THR

2

ASP

PHE

GLN

PHE

LEU

SER

ASP

VAL

3

LEU

ASP

CYS

ARG

ALA

VAL

ARG

SER

ALA

MET

4

ASN

LEU

ARG

ALA

LEU

THR

SER

PHE

GLN

5

VAL

ALA

GLN

TYR

ARG

ASN

ILE

LEU

ASN

ALA

6

SER

LEU

GLN

VAL

ASP

ARG

ASP

ALA

ARG

7

SER

ARG

LEU

MET

ALA

LYS

LEU

ALA

ASP

8

PHE

ALA

VAL

GLU

GLN

GLU

VAL

THR

ALA

GLY

9

ASP

ARG

VAL

ILE

ASP

GLY

LEU

ASP

10

ARG

MET

ALA

HIS

PHE

LYS

ASP

LEU

VAL

11

LEU

VAL

PRO

LEU

THR

LYS

VAL

GLY

12

GLY

SER

ARG

CYS

THR

ILE

CYS

ASP

VAL

13

LYS

GLU

Samples:

sample_1: nsp7a, [U-100% 13C; U-100% 15N], 0.4 – 0.6 mM; H20 90%; D20 10%; potassium phosphate 20 mM; NaCl 50 nM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMRView, Johnson, One Moon Scientific - data analysis

TOPSPIN, Bruker Biospin - collection

VNMR, Varian - collection

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 600 MHz
Varian INOVA 800 MHz
Varian INOVA 600 MHz

PDB	2L8K
DBJ	BAQ56330
EMBL	CAA69186 CAA69187 CAC42774 CAC42775
GB	AAR14191 AAR14192 AAR14200 AAR14201 ABI64071
REF	NP_127506 NP_127507 NP_705589
SP	P19811
AlphaFold	P19811