BMRB Entry 16971

Name: backbone and sidechain 1H, 13C, and 15N assignments for F-plasmid TraI 381-569
Published: 2011-01-06

Click here to enlarge.

PDB ID: 2l8b
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16971
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

backbone and sidechain 1H, 13C, and 15N assignments for F-plasmid TraI 381-569

Deposition date:

2010-06-02

Original release date:

2011-01-06

Authors:

Wright, Nathan; Majumdar, Ananya; Schildbach, Joel

Citation:

Citation: Wright, Nathan; Majumdar, Ananya; Schildbach, Joel. "Chemical shift assignments for F-plasmid TraI (381-569)." Biomol. NMR Assignments 5, 67-70 (2011).
PubMed: 20936510

Assembly members:

Assembly members:
TraI_(381-569), polymer, 189 residues, 20000 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TraI_(381-569): TSGIHVLDELSVRALSRDIM KQNRVTVHPEKSVPRTAGYS DAVSVLAQDRPSLAIVSGQG GAAGQRERVAELVMMAREQG REVQIIAADRRSQMNMKQDE RLSGELITGRRQLLEGMAFT PGSTVIVDQGEKLSLKETLT LLDGAARHNVQVLITDSGQR TGTGSALMAMKDAGVNTYRW QGGEQRPAT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	655
15N chemical shifts	179
1H chemical shifts	1165

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TraI 381-569	1

Entities:

Entity 1, TraI 381-569 189 residues - 20000 Da.

1

THR

SER

GLY

ILE

HIS

VAL

LEU

ASP

GLU

LEU

2

SER

VAL

ARG

ALA

LEU

SER

ARG

ASP

ILE

MET

3

LYS

GLN

ASN

ARG

VAL

THR

VAL

HIS

PRO

GLU

4

LYS

SER

VAL

PRO

ARG

THR

ALA

GLY

TYR

SER

5

ASP

ALA

VAL

SER

VAL

LEU

ALA

GLN

ASP

ARG

6

PRO

SER

LEU

ALA

ILE

VAL

SER

GLY

GLN

GLY

7

GLY

ALA

GLY

GLN

ARG

GLU

ARG

VAL

ALA

8

GLU

LEU

VAL

MET

ALA

ARG

GLU

GLN

GLY

9

ARG

GLU

VAL

GLN

ILE

ALA

ASP

ARG

10

ARG

SER

GLN

MET

ASN

MET

LYS

GLN

ASP

GLU

11

ARG

LEU

SER

GLY

GLU

LEU

ILE

THR

GLY

ARG

12

ARG

GLN

LEU

GLU

GLY

MET

ALA

PHE

THR

13

PRO

GLY

SER

THR

VAL

ILE

VAL

ASP

GLN

GLY

14

GLU

LYS

LEU

SER

LEU

LYS

GLU

THR

LEU

THR

15

LEU

ASP

GLY

ALA

ARG

HIS

ASN

VAL

16

GLN

VAL

LEU

ILE

THR

ASP

SER

GLY

GLN

ARG

17

THR

GLY

THR

GLY

SER

ALA

LEU

MET

ALA

MET

18

LYS

ASP

ALA

GLY

VAL

ASN

THR

TYR

ARG

TRP

19

GLN

GLY

GLU

GLN

ARG

PRO

ALA

THR

Samples:

sample_1: TraI (381-569), [U-100% 13C; U-100% 15N], 300 – 600 uM; D2O 10%; H2O 90%; TRIS 20 mM; sodium chloride 25 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.2; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

NMR spectrometers:

Bruker Avance 600 MHz
Varian INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks