BMRB Entry 16947

Name: Backbone structure of the membrane domain of E. coli histidine kinase receptor ArcB
Published: 2010-06-15

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PDB ID: 2ksd
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16947
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone structure of the membrane domain of E. coli histidine kinase receptor ArcB

Deposition date:

2010-05-24

Original release date:

2010-06-15

Authors:

Maslennikov, Innokentiy; Klammt, Christian; Hwang, Eunha; Kefala, Georgia; Kwiatkowski, Witek; Jeon, Young Ho; Choe, Senyon

Citation:

Citation: Maslennikov, Innokentiy; Klammt, Christian; Hwang, Eunha; Kefala, Georgia; Okamura, Mizuki; Esquivies, Luis; Mors, Karsten; Glaubitz, Clemens; Kwiatkowski, Witek; Jeon, Young Ho; Choe, Senyon. "Membrane domain structures of three classes of histidine kinase receptors by cell-free expression and rapid NMR analysis." Proc. Natl. Acad. Sci. U.S.A. 107, 10902-10907 (2010).
PubMed: 20498088

Assembly members:

Assembly members:
ArcB, polymer, 115 residues, 8483.369 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: pIVEX2.3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ArcB: MKQIRLLAQYYVDLMMKLGL VRFSMLLALALVVLAIVVQM AVTMVLHGQVESIDVIRSIF FGLLITPWAVYFLSVVVEQL EESRQRLSRLVQKLEEMRER DLSLNVQLKDNIAQL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	317
15N chemical shifts	108
1H chemical shifts	491

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ArcB	1

Entities:

Entity 1, ArcB 115 residues - 8483.369 Da.

The membrane domain of ArcB

1

MET

LYS

GLN

ILE

ARG

LEU

ALA

GLN

TYR

2

TYR

VAL

ASP

LEU

MET

LYS

LEU

GLY

LEU

3

VAL

ARG

PHE

SER

MET

LEU

ALA

LEU

ALA

4

LEU

VAL

LEU

ALA

ILE

VAL

GLN

MET

5

ALA

VAL

THR

MET

VAL

LEU

HIS

GLY

GLN

VAL

6

GLU

SER

ILE

ASP

VAL

ILE

ARG

SER

ILE

PHE

7

PHE

GLY

LEU

ILE

THR

PRO

TRP

ALA

VAL

8

TYR

PHE

LEU

SER

VAL

GLU

GLN

LEU

9

GLU

SER

ARG

GLN

ARG

LEU

SER

ARG

LEU

10

VAL

GLN

LYS

LEU

GLU

MET

ARG

GLU

ARG

11

ASP

LEU

SER

LEU

ASN

VAL

GLN

LEU

LYS

ASP

12

ASN

ILE

ALA

GLN

LEU

Samples:

sample_EC: DSS 0.5 mM; sodium acetate 20 mM; 1-myristoyl-2-hydroxy-sn-glycero-3-[phospho-rac-(1-glycerol)] 200 mM; sodium chloride 10 mM; Protein, [U-98% 13C; U-98% 15N], 0.5 mM; H2O 95%; D2O 5%

sample_CF: DSS 0.5 mM; 1-myristoyl-2-hydroxy-sn-glycero-3-[phospho-rac-(1-glycerol)] 100 mM; Mes-BisTris 20 mM; Protein, [U-99% 13C; U-99% 15N], 0.3 mM; H2O 95%; D2O 5%

sample_conditions_EC: pH: 5.5; pressure: 1 atm; temperature: 318 K

sample_conditions_CF: pH: 5.5; pressure: 1 atm; temperature: 318 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_EC	isotropic	sample_conditions_EC
2D 1H-15N HSQC	sample_CF	isotropic	sample_conditions_CF
3D 1H-15N NOESY	sample_EC	isotropic	sample_conditions_EC
3D 1H-15N NOESY	sample_CF	isotropic	sample_conditions_CF
3D HNCA	sample_CF	isotropic	sample_conditions_CF
3D HNCO	sample_CF	isotropic	sample_conditions_CF
3D HNCACB	sample_CF	isotropic	sample_conditions_CF
3D HN(CO)CA	sample_CF	isotropic	sample_conditions_CF
3D HN(CA)CO	sample_CF	isotropic	sample_conditions_CF

Software:

CYANA v1.0.6, Guntert, Mumenthaler and Wuthrich - structure solution

TOPSPIN v2.1, Bruker Biospin - collection

CARA v1.8.4, Keller and Wuthrich - data analysis, peak picking

Molmol v2K.2, Koradi, Billeter and Wuthrich - structure visualization and analysis

NMR spectrometers:

Bruker Avance 900 MHz
Bruker Avance 700 MHz

PDB	2KSD
DBJ	BAB37512 BAE77254 BAG79018 BAH65394 BAI27488
EMBL	CAA37397 CAD07845 CAP77670 CAQ33543 CAQ90680
GB	AAA58012 AAG58344 AAL22197 AAN44715 AAN82410
PIR	AG0906
REF	NP_312116 NP_457707 NP_462238 NP_709008 WP_000809745
SP	P0AEC3 P0AEC4 P58363
AlphaFold	P0AEC3 P0AEC4 P58363