BMRB Entry 16937

Title:
apoWCBD4-6
Deposition date:
2010-05-18
Original release date:
2010-09-08
Authors:
Fatemi, Negah
Citation:

Citation: Fatemi, Negah; Korzhnev, Dmitry; Velyvis, Algirdas; Sarkar, Bibudhendra; Forman-Kay, Julie. "NMR characterization of copper-binding domains 4-6 of ATP7B ."  Biochemistry 49, 8468-8477 (2010).
PubMed: 20799727

Assembly members:

Assembly members:
apoWCBD4-6, polymer, 281 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET SUMO

Data sets:
Data typeCount
13C chemical shifts667
15N chemical shifts227
1H chemical shifts227

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apoWCBD4-61

Entities:

Entity 1, apoWCBD4-6 281 residues - Formula weight is not available

1   GLYPROLEUGLYSERTHRCYSSERTHRTHR
2   LEUILEALAILEALAGLYMETTHRCYSALA
3   SERCYSVALHISSERILEGLUGLYMETILE
4   SERGLNLEUGLUGLYVALGLNGLNILESER
5   VALSERLEUALAGLUGLYTHRALATHRVAL
6   LEUTYRASNPROALAVALILESERPROGLU
7   GLULEUARGALAALAILEGLUASPMETGLY
8   PHEGLUALASERVALVALSERGLUSERCYS
9   SERTHRASNPROLEUGLYASNHISSERALA
10   GLYASNSERMETVALGLNTHRTHRASPGLY
11   THRPROTHRSERLEUGLNGLUVALALAPRO
12   HISTHRGLYARGLEUPROALAASNHISALA
13   PROASPILELEUALALYSSERPROGLNSER
14   THRARGALAVALALAPROGLNLYSCYSPHE
15   LEUGLNILELYSGLYMETTHRCYSALASER
16   CYSVALSERASNILEGLUARGASNLEUGLN
17   LYSGLUALAGLYVALLEUSERVALLEUVAL
18   ALALEUMETALAGLYLYSALAGLUILELYS
19   TYRASPPROGLUVALILEGLNPROLEUGLU
20   ILEALAGLNPHEILEGLNASPLEUGLYPHE
21   GLUALAALAVALMETGLUASPTYRALAGLY
22   SERASPGLYASNILEGLULEUTHRILETHR
23   GLYMETTHRCYSALASERCYSVALHISASN
24   ILEGLUSERLYSLEUTHRARGTHRASNGLY
25   ILETHRTYRALASERVALALALEUALATHR
26   SERLYSALALEUVALLYSPHEASPPROGLU
27   ILEILEGLYPROARGASPILEILELYSILE
28   ILEGLUGLUILEGLYPHEHISALASERLEU
29   ALA

Samples:

sample_1: apoWCBD4-6, [U-100% 13C; U-100% 15N], 450 – 500 uM; D2O 100%

sample_conditions_1: ionic strength: 0.19 M; pH: 6; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D H(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
(H)CC(CO)NH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian Unity INOVA 500 MHz
  • Varian Unity INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks