BMRB Entry 16926

Click here to enlarge.

PDB ID: 2kxx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16926
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
NMR Structure of Escherichia coli BamE, a Lipoprotein Component of the beta-Barrel Assembly Machinery Complex
Deposition date:
2010-05-13
Original release date:
2011-01-06
Authors:
Kim, Kelly; Okon, Mark; Escobar, Eric; Kang, Hyun-Seo; McIntosh, Lawrence; Paetzel, Mark
Citation:

Citation: Kim, Kelly; Kang, Hyun-Seo; Okon, Mark; Escobar-Cabrera, Eric; McIntosh, Lawrence; Paetzel, Mark. "Structural Characterization of Escherichia coli BamE, a Lipoprotein Component of the -Barrel Assembly Machinery Complex."  Biochemistry 50, 1081-1090 (2011).
PubMed: 21207987

Assembly members:

Assembly members:
E._coli_lipoprotein, polymer, 97 residues, 8072.073 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E._coli_lipoprotein: GSHMSTLERVVYRPDINQGN YLTANDVSKIRVGMTQQQVA YALGTPLMSDPFGTNTWFYV FRQQPGHEGVTQQTLTLTFN SSGVLTNIDNKPALSGN

Data sets:
Data typeCount
13C chemical shifts236
15N chemical shifts92
1H chemical shifts544

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E. coli lipoprotein1

Entities:

Entity 1, E. coli lipoprotein 97 residues - 8072.073 Da.

First four residues (GSHM) are remainder of thrombin-cleaved 6-His tag. This structure represents the full length mature BamE protein without its lipidated cysteine (Cys20).

1   GLYSERHISMETSERTHRLEUGLUARGVAL
2   VALTYRARGPROASPILEASNGLNGLYASN
3   TYRLEUTHRALAASNASPVALSERLYSILE
4   ARGVALGLYMETTHRGLNGLNGLNVALALA
5   TYRALALEUGLYTHRPROLEUMETSERASP
6   PROPHEGLYTHRASNTHRTRPPHETYRVAL
7   PHEARGGLNGLNPROGLYHISGLUGLYVAL
8   THRGLNGLNTHRLEUTHRLEUTHRPHEASN
9   SERSERGLYVALLEUTHRASNILEASPASN
10   LYSPROALALEUSERGLYASN

Related Database Links:

BMRB 16424
PDB 2KM7 2KXX 2YH9
DBJ BAA16502 BAB36902 BAG78424 BAI26856 BAI31942
EMBL CAH23267 CAP77059 CAQ32986 CAQ88015 CAQ99565
GB AAA79787 AAC75666 AAG57727 AAN44171 AAN81589
REF NP_311506 NP_417107 NP_708464 WP_001203434 WP_001203436
SP P0A937 P0A938 P0A939 Q32CX2
AlphaFold P0A937 P0A938 P0A939 Q32CX2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks