BMRB Entry 16918

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16918

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Title:
Mg(2+)-bound receiver domain of sensor histidine kinase CKI1RD of Arabidopsis thaliana
Deposition date:
2010-05-10
Original release date:
2011-05-23
Authors:
Pekarova, Blanka; Klumpler, Tomas; Triskova, Olga; Horak, Jakub; Jansen, Severine; Dopitova, Radka; Motackova, Veronika; Nejedla, Eliska; Zidek, Lukas; Marek, Jaromir; Sklenar, Vladimir; Hejatko, Jan; Janda, Lubomir
Citation:

Citation: Pekarova, Blanka; Klumpler, Toma; Tiskova, Olga; Horak, Jakub; Jansen, Severine; Dopitova, Radka; Borkovcova, Petra; Papoukova, Veronika; Nejedla, Elika; Sklena, Vladimir; Marek, Jaromir; Zidek, Luka; Hejatko, Jan; Janda, Lubomir. "Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana."  Plant J. 67, 827-839 (2011).
PubMed: 21569135

Assembly members:

Assembly members:
CKI1RD, polymer, 207 residues, Formula weight is not available
MG, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGBKT7-DEST

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CKI1RD: MGSSHHHHHHSSGLVPRGSH MASTDSESETRVKSVRTGRK PIGNPEDEQETSKPSDDEFL RGKRVLVVDDNFISRKVATG KLKKMGVSEVEQCDSGKEAL RLVTEGLTQREEQGSVDKLP FDYIFMDCQMPEMDGYEATR EIRKVEKSYGVRTPIIAVSG HDPGSEEARETIQAGMDAFL DKSLNQLANVIREIESKRHL EHHHHHH

Data sets:
Data typeCount
13C chemical shifts325
15N chemical shifts161
1H chemical shifts161
General relaxation values291
chemical shift anisotropy values97
heteronuclear NOE values97
order parameters97
tensors97

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CKI1RD1
2MG2

Entities:

Entity 1, CKI1RD 207 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METALASERTHRASPSERGLUSERGLUTHR
4   ARGVALLYSSERVALARGTHRGLYARGLYS
5   PROILEGLYASNPROGLUASPGLUGLNGLU
6   THRSERLYSPROSERASPASPGLUPHELEU
7   ARGGLYLYSARGVALLEUVALVALASPASP
8   ASNPHEILESERARGLYSVALALATHRGLY
9   LYSLEULYSLYSMETGLYVALSERGLUVAL
10   GLUGLNCYSASPSERGLYLYSGLUALALEU
11   ARGLEUVALTHRGLUGLYLEUTHRGLNARG
12   GLUGLUGLNGLYSERVALASPLYSLEUPRO
13   PHEASPTYRILEPHEMETASPCYSGLNMET
14   PROGLUMETASPGLYTYRGLUALATHRARG
15   GLUILEARGLYSVALGLULYSSERTYRGLY
16   VALARGTHRPROILEILEALAVALSERGLY
17   HISASPPROGLYSERGLUGLUALAARGGLU
18   THRILEGLNALAGLYMETASPALAPHELEU
19   ASPLYSSERLEUASNGLNLEUALAASNVAL
20   ILEARGGLUILEGLUSERLYSARGHISLEU
21   GLUHISHISHISHISHISHIS

Entity 2, MG - Mg - 24.305 Da.

1   MG

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