BMRB Entry 16914

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16914

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Title:
Solution strcture of UBX domain of human UBXD2 protein
Deposition date:
2010-05-06
Original release date:
2012-08-02
Authors:
Wu, Qinglin; Huang, Hongda; Zhang, Jiahai; Hu, Qi; Wu, Jihui; Shi, Yunyu
Citation:

Citation: Wu, Qinglin; Huang, Hongda; Zhang, Jiahai; Hu, Qi; Wu, Jihui; Shi, Yunyu. "Solution strcture of UBX domain of human UBXD2 protein"  .

Assembly members:

Assembly members:
UBX domain of human UBXD2, polymer, 90 residues, 10112.486 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UBX domain of human UBXD2: MGHHHHHHMSTVARIQFRLP DGSSFTNQFPSDAPLEEARQ FAAQTVGNTYGNFSLATMFP RREFTKEDYKKKLLDLELAP SASVVLLPAG

Data sets:
Data typeCount
13C chemical shifts311
15N chemical shifts79
1H chemical shifts535

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UBX1

Entities:

Entity 1, UBX 90 residues - 10112.486 Da.

1   METGLYHISHISHISHISHISHISMETSER
2   THRVALALAARGILEGLNPHEARGLEUPRO
3   ASPGLYSERSERPHETHRASNGLNPHEPRO
4   SERASPALAPROLEUGLUGLUALAARGGLN
5   PHEALAALAGLNTHRVALGLYASNTHRTYR
6   GLYASNPHESERLEUALATHRMETPHEPRO
7   ARGARGGLUPHETHRLYSGLUASPTYRLYS
8   LYSLYSLEULEUASPLEUGLULEUALAPRO
9   SERALASERVALVALLEULEUPROALAGLY

Samples:

sample_1: UBX, [U-100% 13C; U-100% 15N], 0.8 mM; PBS 50 mM; NaCl 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB
REF XP_003794437 XP_004032644 XP_006996353 XP_007129544 XP_008831885

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks