BMRB Entry 16913

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PDB ID: 2kxg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16913
MolProbity Validation Chart

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Title:
The solution structure of the squash aspartic acid proteinase inhibitor (SQAPI)
Deposition date:
2010-05-05
Original release date:
2010-07-26
Authors:
Headey, Stephen; MacAskill, Ursula; Wright, Michelle; Claridge, Jolyon; Edwards, Patrick; Farley, Peter; Christeller, John; Laing, William; Pascal, Steven
Citation:

Citation: Headey, Stephen; Macaskill, Ursula; Wright, Michele; Claridge, Jolyon; Edwards, Patrick; Farley, Peter; Christeller, John; Laing, William; Pascal, Steven. "Solution structure of the squash aspartic acid proteinase inhibitor (SQAPI) and mutational analysis of pepsin inhibition."  J. Biol. Chem. 285, 27019-27025 (2010).
PubMed: 20538608

Assembly members:

Assembly members:
SQAPI, polymer, 95 residues, 10421.041 Da.

Natural source:

Natural source:   Common Name: winter squash   Taxonomy ID: 3661   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Cucurbita maxima

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSETA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SQAPI: GPGPAIGEVIGISVNDPRVK EIAEFALKQHAEQNLILAGV DAGQIIKGIPHWDNYYNLIL SAKHSPHEFSKFYNVVVLEK ASDNSLKLVAFVPLF

Data sets:
Data typeCount
13C chemical shifts421
1H chemical shifts674

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1SQAPI1

Entities:

Entity 1, SQAPI 95 residues - 10421.041 Da.

1   GLYPROGLYPROALAILEGLYGLUVALILE
2   GLYILESERVALASNASPPROARGVALLYS
3   GLUILEALAGLUPHEALALEULYSGLNHIS
4   ALAGLUGLNASNLEUILELEUALAGLYVAL
5   ASPALAGLYGLNILEILELYSGLYILEPRO
6   HISTRPASPASNTYRTYRASNLEUILELEU
7   SERALALYSHISSERPROHISGLUPHESER
8   LYSPHETYRASNVALVALVALLEUGLULYS
9   ALASERASPASNSERLEULYSLEUVALALA
10   PHEVALPROLEUPHE

Related Database Links:

BMRB 15483
PDB 2KXG
GB AAC39473 AAT67162 ABB96300 ABB96305 ABB96307