BMRB Entry 16905

Name: Sma0114
Published: 2010-10-27

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PDB ID: 2lpm
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16905
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sma0114

Deposition date:

2010-04-29

Original release date:

2010-10-27

Authors:

Sheftic, Sarah; Gage, Dan; Alexandrescu, Andrei

Citation:

Citation: Sheftic, Sarah; Garcia, Preston; Robinson, Victoria; Gage, Daniel; Alexandrescu, Andrei. "NMR assignments for the Sinorhizobium meliloti response regulator Sma0114." Biomol. NMR Assignments 5, 55-58 (2011).
PubMed: 20936511

Assembly members:

Assembly members:
Sma0114, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Sinorhizobium meliloti Taxonomy ID: 382 Superkingdom: Bacteria Kingdom: not available Genus/species: Sinorhizobium meliloti

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sma0114: GSHMTERRLRVLVVEDESMI AMLIEDTLCELGHEVAATAS RMQEALDIARKGQFDIAIID VNLDGEPSYPVADILAERNV PFIFATGYGSKGLDTRYSNI PLLTKPFLDSELEAVLVQIS KEV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
coupling_constants
- coupling_constant_list_1

Data type	Count
13C chemical shifts	478
15N chemical shifts	119
1H chemical shifts	660
coupling constants	78

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	response regulator	1

Entities:

Entity 1, response regulator 123 residues - Formula weight is not available

1

GLY

SER

HIS

MET

THR

GLU

ARG

LEU

ARG

2

VAL

LEU

VAL

GLU

ASP

GLU

SER

MET

ILE

3

ALA

MET

LEU

ILE

GLU

ASP

THR

LEU

CYS

GLU

4

LEU

GLY

HIS

GLU

VAL

ALA

THR

ALA

SER

5

ARG

MET

GLN

GLU

ALA

LEU

ASP

ILE

ALA

ARG

6

LYS

GLY

GLN

PHE

ASP

ILE

ALA

ILE

ASP

7

VAL

ASN

LEU

ASP

GLY

GLU

PRO

SER

TYR

PRO

8

VAL

ALA

ASP

ILE

LEU

ALA

GLU

ARG

ASN

VAL

9

PRO

PHE

ILE

PHE

ALA

THR

GLY

TYR

GLY

SER

10

LYS

GLY

LEU

ASP

THR

ARG

TYR

SER

ASN

ILE

11

PRO

LEU

THR

LYS

PRO

PHE

LEU

ASP

SER

12

GLU

LEU

GLU

ALA

VAL

LEU

VAL

GLN

ILE

SER

13

LYS

GLU

VAL

Samples:

sample_1: Sma114, [U-100% 15N], 500 uM; H2O 90%; D2O 10%; sodium phosphate 50 mM; DTT 1 mM

sample_2: Sma114, [U-100% 13C; U-100% 15N], 500 uM; D2O 100%; sodium phosphate 50 mM; DTT 1 mM

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HN(CO)CA	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HN(CA)CO	sample_2	isotropic	sample_conditions_1
HSQC TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HNHB	sample_2	isotropic	sample_conditions_1
3D HNHA	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1

Software:

FELIX, Accelrys Software Inc. - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	19286
PDB	2LPM 2M98
EMBL	CCM71308 CDH82688
GB	AAK64716 AEG06710 AEG57042 AEH82764 AGG69741
REF	NP_435304 WP_010967059 WP_033048070 WP_046066705