BMRB Entry 16890

Name: Structure of G61-101
Published: 2010-07-27

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16890

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of G61-101

Deposition date:

2010-04-22

Original release date:

2010-07-27

Authors:

S., Rishikesan; Gruber, Gerhard

Citation:

Citation: Rishikesan, Sankaranarayanan; Manimekalai, Malathy; Gruber, Gerhard. "The NMR solution structure of subunit G (G(61)(-)(101)) of the eukaryotic V1VO ATPase from Saccharomyces cerevisiae." Biochim. Biophys. Acta 1798, 1961-1968 (2010).
PubMed: 20599533

Assembly members:

Assembly members:
G61-101, polymer, 43 residues, 4604.364 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
G61-101: MDNAGGVGELEKKAEAGVQG ELAEIKKIAEKKKDDVVKIL IET

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	122
15N chemical shifts	43
1H chemical shifts	296

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	G61-101	1

Entities:

Entity 1, G61-101 43 residues - 4604.364 Da.

1

MET

ASP

ASN

ALA

GLY

VAL

GLY

GLU

LEU

2

GLU

LYS

ALA

GLU

ALA

GLY

VAL

GLN

GLY

3

GLU

LEU

ALA

GLU

ILE

LYS

ILE

ALA

GLU

4

LYS

ASP

VAL

LYS

ILE

LEU

5

ILE

GLU

THR

Samples:

sample_1: D2O 7%; TFE, [U-100% 2H], 30%; sodium phosphate 25 mM; sodium chloride 200 mM; G61-101, [U-100% 13C; U-100% 15N], 1 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v2.1, Goddard - chemical shift assignment

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - chemical shift calculation

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

Bruker Avance 600 MHz

PDB	2KWY 3J9T 3J9U 3J9V 4DL0 4EFA
DBJ	GAA23755
EMBL	CAY80326
GB	AAA74570 AAB68921 AHY77737 AJP39136 AJU16333
REF	NP_011905
SP	P48836
TPG	DAA06731
AlphaFold	P48836