BMRB Entry 16884

Name: 1H, 13C and 15N Chemical Shift assignments for RRM3 of Brunol-3.
Published: 2011-12-15

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PDB ID: 2my8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16884
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Chemical Shift assignments for RRM3 of Brunol-3.

Deposition date:

2010-04-20

Original release date:

2011-12-15

Authors:

Kashyap, Maruthi; Bhatt, Harshesh; Bhavesh, Neel Sarovar

Citation:

Citation: Kashyap, Maruthi; Bhatt, Harshesh; Bhavesh, Neel Sarovar. "1H, 13C and 15N NMR assignments of RNA recognizing motifs 1 and 2 of BRUNOL-3 protein from human involved in myotonic dystrophy" Biomol. NMR Assignments 4, 143-145 (2010).
PubMed: 20443086

Assembly members:

Assembly members:
RRM3, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RRM3: GSHMQKEGPEGANLFIYHLP QEFGDQDILQMFMPFGNVIS AKVFIDKQTNLSKCFGFVSY DNPVSAQAAIQAMNGFQIGM KRLKVQLKRSKNDSKPY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	395
15N chemical shifts	107
1H chemical shifts	562

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RRM3	1

Entities:

Entity 1, RRM3 97 residues - Formula weight is not available

1

GLY

SER

HIS

MET

GLN

LYS

GLU

GLY

PRO

GLU

2

GLY

ALA

ASN

LEU

PHE

ILE

TYR

HIS

LEU

PRO

3

GLN

GLU

PHE

GLY

ASP

GLN

ASP

ILE

LEU

GLN

4

MET

PHE

MET

PRO

PHE

GLY

ASN

VAL

ILE

SER

5

ALA

LYS

VAL

PHE

ILE

ASP

LYS

GLN

THR

ASN

6

LEU

SER

LYS

CYS

PHE

GLY

PHE

VAL

SER

TYR

7

ASP

ASN

PRO

VAL

SER

ALA

GLN

ALA

ILE

8

GLN

ALA

MET

ASN

GLY

PHE

GLN

ILE

GLY

MET

9

LYS

ARG

LEU

LYS

VAL

GLN

LEU

LYS

ARG

SER

10

LYS

ASN

ASP

SER

LYS

PRO

TYR

Samples:

sample_1: BRUNOL-3, [U-13C; U-15N], 1 – 1.2 mM; sodium phosphate 20 ± 0.5 mM; sodium chloride 50 ± 0.5 mM; sodium azide 0.1%; D2O, [U-99% 2H], 5%; D2O 95%

sample_conditions_1: pH: 6.5; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HNN	sample_1	isotropic	sample_conditions_1
3D HN(C)N	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 700 MHz

BMRB	19685
PDB	2MY7 2MY8 4LJM
DBJ	BAE36341 BAG58428 BAG59778 BAG63913
EMBL	CAF96701 CDQ91280
GB	AAF89096 AAH45035 AAI36077 AAP57761 AAP57762
REF	NP_001079593 NP_001096417 NP_001279263 XP_004574118 XP_004574119
SP	A4IIM2 Q7T2T1 Q7ZXE2
AlphaFold	A4IIM2 Q7T2T1 Q7ZXE2