BMRB Entry 16874

Name: The solution structure of rat Lipocalin-type Prostaglandin D Synthase
Published: 2010-05-17

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16874

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The solution structure of rat Lipocalin-type Prostaglandin D Synthase

Deposition date:

2010-04-16

Original release date:

2010-05-17

Authors:

Liu, Jiafu; Lin, Donghai

Citation:

Citation: Liu, Jiafu; Guo, Chenyun; Yao, Yihe; Lin, Donghai. "The solution structure and dynamics of rat Lipocalin-Type Prostaglandin D Synthase" Biochimie 90, 1637-1646 (2008).
PubMed: 18590794

Assembly members:

Assembly members:
rL-PGDS, polymer, 165 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rL-PGDS: QGHDTVQPNFQQDKFLGRWY SAGLASNSSWFREKKELLFM CQTVVAPSTEGGLNLTSTFL RKNQCETKVMVLQPAGVPGQ YTYNSPHWGSFHSLSVVETD YDEYAFLFSKGTKGPGQDFR MATLYSRAQLLKEELKEKFI TFSKDQGLTEEDIVFLPQPD KCIQE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	599
15N chemical shifts	153
1H chemical shifts	865

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rat Lipocalin-Type Prostaglandin D Synthase	1

Entities:

Entity 1, rat Lipocalin-Type Prostaglandin D Synthase 165 residues - Formula weight is not available

1

GLN

GLY

HIS

ASP

THR

VAL

GLN

PRO

ASN

PHE

2

GLN

ASP

LYS

PHE

LEU

GLY

ARG

TRP

TYR

3

SER

ALA

GLY

LEU

ALA

SER

ASN

SER

TRP

4

PHE

ARG

GLU

LYS

GLU

LEU

PHE

MET

5

CYS

GLN

THR

VAL

ALA

PRO

SER

THR

GLU

6

GLY

LEU

ASN

LEU

THR

SER

THR

PHE

LEU

7

ARG

LYS

ASN

GLN

CYS

GLU

THR

LYS

VAL

MET

8

VAL

LEU

GLN

PRO

ALA

GLY

VAL

PRO

GLY

GLN

9

TYR

THR

TYR

ASN

SER

PRO

HIS

TRP

GLY

SER

10

PHE

HIS

SER

LEU

SER

VAL

GLU

THR

ASP

11

TYR

ASP

GLU

TYR

ALA

PHE

LEU

PHE

SER

LYS

12

GLY

THR

LYS

GLY

PRO

GLY

GLN

ASP

PHE

ARG

13

MET

ALA

THR

LEU

TYR

SER

ARG

ALA

GLN

LEU

14

LEU

LYS

GLU

LEU

LYS

GLU

LYS

PHE

ILE

15

THR

PHE

SER

LYS

ASP

GLN

GLY

LEU

THR

GLU

16

GLU

ASP

ILE

VAL

PHE

LEU

PRO

GLN

PRO

ASP

17

LYS

CYS

ILE

GLN

GLU

Samples:

sample_1: rL-PGDS, [U-100% 13C; U-100% 15N], 1.0 mM; H2O 90%; D2O 10%; sodium phosphate 15 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 0.200 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

GB	AAA41839 AAA41840 EDL93575 EDL93576 EDL93577
REF	NP_037147
SP	P22057
AlphaFold	P22057