BMRB Entry 16872

Title:
Mcm10 C-terminal DNA binding domain
Deposition date:
2010-04-15
Original release date:
2010-05-26
Authors:
Robertson, Patrick; Chagot, Benjamin; Chazin, Walter; Eichman, Brandt
Citation:

Citation: Robertson, Patrick; Chagot, Benjamin; Chazin, Walter; Eichman, Brandt. "Solution NMR structure of the C-terminal DNA binding domain of Mcm10 reveals a conserved MCM motif."  J. Biol. Chem. 285, 22942-22949 (2010).
PubMed: 20489205

Assembly members:

Assembly members:
Mcm10, polymer, 92 residues, 10596.545 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32a-PPS

Data sets:
Data typeCount
13C chemical shifts282
15N chemical shifts98
1H chemical shifts645

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mcm101
2ZINC ION 12
3ZINC ION 22

Entities:

Entity 1, Mcm10 92 residues - 10596.545 Da.

First four residues are a non-native affinity tag residual

1   GLYPROMETGLYMETGLNSERILEARGGLU
2   GLNSERCYSARGVALVALTHRCYSLYSTHR
3   CYSLYSTYRTHRHISPHELYSPROLYSGLU
4   THRCYSVALSERGLUASNHISASPPHEHIS
5   TRPHISASNGLYVALLYSARGPHEPHELYS
6   CYSPROCYSGLYASNARGTHRILESERLEU
7   ASPARGLEUPROLYSLYSHISCYSSERTHR
8   CYSGLYLEUPHELYSTRPGLUARGVALGLY
9   METLEULYSGLULYSTHRGLYPROLYSLEU
10   GLYGLY

Entity 2, ZINC ION 1 - Zn - 65.409 Da.

1   ZN

Samples:

N-enriched_(920): entity_1, [U-100% 15N], 920 uM; sodium phosphate 25 mM; sodium chloride 100 mM; D2O 5%; H2O 95%

N-enriched_(300): entity_1, [U-100% 15N], 300 uM; sodium phosphate 25 mM; sodium chloride 100 mM; D2O 5%; H2O 95%

C-enriched_(920): entity_1, [U-100% 13C], 920 uM; sodium phosphate 25 mM; sodium chloride 100 mM; D2O 5%; H2O 95%

NC-enriched_(920): entity_1, [U-100% 13C; U-100% 15N], 920 uM; sodium phosphate 25 mM; sodium chloride 100 mM; D2O 5%; H2O 95%

natural: entity_1 920 uM; sodium phosphate 25 mM; sodium chloride 100 mM; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCN-enriched_(300)isotropicsample_conditions_1
2D 1H-13C HSQCC-enriched_(920)isotropicsample_conditions_1
2D 1H-1H TOCSYnaturalisotropicsample_conditions_1
2D 1H-1H NOESYnaturalisotropicsample_conditions_1
2D 1H-1H COSYnaturalisotropicsample_conditions_1
3D CBCA(CO)NHNC-enriched_(920)isotropicsample_conditions_1
3D HNCONC-enriched_(920)isotropicsample_conditions_1
3D HNCACBNC-enriched_(920)isotropicsample_conditions_1
3D HBHA(CO)NHNC-enriched_(920)isotropicsample_conditions_1
3D HNHAN-enriched_(920)isotropicsample_conditions_1
3D H(CCO)NHNC-enriched_(920)isotropicsample_conditions_1
3D C(CO)NHNC-enriched_(920)isotropicsample_conditions_1
3D 1H-15N NOESYN-enriched_(920)isotropicsample_conditions_1
3D 1H-13C NOESYC-enriched_(920)isotropicsample_conditions_1
3D (HB)CB(CGCD)HDN-enriched_(920)isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

SPARKY, Goddard - peak picking

TOPSPIN, Bruker Biospin - collection

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
GB AAG33858 AAH70548 AAH90220
REF NP_001082047
SP Q5EAW4
AlphaFold Q5EAW4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks