BMRB Entry 16869

Name: Backbone assignment of the catalytic core of a Y-family DNA polymerase
Published: 2010-07-27

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16869

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of the catalytic core of a Y-family DNA polymerase

Deposition date:

2010-04-15

Original release date:

2010-07-27

Authors:

Ma, Dejian; Fowler, Jason; Yuan, Chunhua; Suo, Zucai

Citation:

Citation: Ma, Dejian; Fowler, Jason; Yuan, Chunhua; Suo, Zucai. "Backbone assignment of the catalytic core of a Y-family DNA polymerase." Biomol. NMR Assignments 4, 207-209 (2010).
PubMed: 20582735

Assembly members:

Assembly members:
Dpo4, polymer, 250 residues, 27957.5 Da.

Natural source:

Natural source: Common Name: Sulfolobus solfataricus Taxonomy ID: 2287 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus solfataricus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dpo4: MGSSHHHHHHSSGLVPRGSH MIVLFVDFDYFYAQVEEVLN PSLKGKPVVVCVFSGRFEDS GAVATANYEARKFGVKAGIP IVEAKKILPNAVYLPMRKEV YQQVSSRIMNLLREYSEKIE IASIDEAYLDISDKVRDYRE AYNLGLEIKNKILEKEKITV TVGISKNKVFAKIAADMAKP NGIKVIDDEEVKRLIRELDI ADVPGIGNITAEKLKKLGIN KLVDTLSIEFDKLKGMIGEA KAKYLISLAR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	513
15N chemical shifts	185
1H chemical shifts	185

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	catalytic core of Dpo4	1

Entities:

Entity 1, catalytic core of Dpo4 250 residues - 27957.5 Da.

First 20 residues, -19 to 0, represent a non-native affinity tag.

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	ILE	VAL	LEU	PHE	VAL	ASP	PHE	ASP	TYR
4	PHE	TYR	ALA	GLN	VAL	GLU	GLU	VAL	LEU	ASN
5	PRO	SER	LEU	LYS	GLY	LYS	PRO	VAL	VAL	VAL
6	CYS	VAL	PHE	SER	GLY	ARG	PHE	GLU	ASP	SER
7	GLY	ALA	VAL	ALA	THR	ALA	ASN	TYR	GLU	ALA
8	ARG	LYS	PHE	GLY	VAL	LYS	ALA	GLY	ILE	PRO
9	ILE	VAL	GLU	ALA	LYS	LYS	ILE	LEU	PRO	ASN
10	ALA	VAL	TYR	LEU	PRO	MET	ARG	LYS	GLU	VAL
11	TYR	GLN	GLN	VAL	SER	SER	ARG	ILE	MET	ASN
12	LEU	LEU	ARG	GLU	TYR	SER	GLU	LYS	ILE	GLU
13	ILE	ALA	SER	ILE	ASP	GLU	ALA	TYR	LEU	ASP
14	ILE	SER	ASP	LYS	VAL	ARG	ASP	TYR	ARG	GLU
15	ALA	TYR	ASN	LEU	GLY	LEU	GLU	ILE	LYS	ASN
16	LYS	ILE	LEU	GLU	LYS	GLU	LYS	ILE	THR	VAL
17	THR	VAL	GLY	ILE	SER	LYS	ASN	LYS	VAL	PHE
18	ALA	LYS	ILE	ALA	ALA	ASP	MET	ALA	LYS	PRO
19	ASN	GLY	ILE	LYS	VAL	ILE	ASP	ASP	GLU	GLU
20	VAL	LYS	ARG	LEU	ILE	ARG	GLU	LEU	ASP	ILE
21	ALA	ASP	VAL	PRO	GLY	ILE	GLY	ASN	ILE	THR
22	ALA	GLU	LYS	LEU	LYS	LYS	LEU	GLY	ILE	ASN
23	LYS	LEU	VAL	ASP	THR	LEU	SER	ILE	GLU	PHE
24	ASP	LYS	LEU	LYS	GLY	MET	ILE	GLY	GLU	ALA
25	LYS	ALA	LYS	TYR	LEU	ILE	SER	LEU	ALA	ARG

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

PDB	1JX4 1JXL 1N48 1N56 1RYR 1RYS 1S0M 1S0N 1S0O 1S10 1S97 1S9F 2AGO 2AGP 2AGQ 2ASD 2ASJ 2ASL 2ATL 2AU0 2IA6 2IBK 2IMW 2R8G 2R8H 2R8I 2RDI 2RDJ 3FDS 3GII 3GIJ 3GIK 3GIL 3GIM 3KHG 3KHH 3KHL 3KHR 3M9M 3M9N 3M9O 3PR4 3PR5 3QZ7 3QZ8 3RAQ 3RAX 3RB0 3RB3 3RB4 3RB6 3RBD 3RBE 3T5H 3T5J 3T5K 3T5L 4F4Z 4FBT 4FBU 4G3I 4QW8 4QW9 4QWA 4QWB 4QWC 4QWD 4QWE 4RUA 4RUC 4RZR 4TQR
GB	AAK42588 ACX90547 AKA72680 AKA75380 AKA78071
REF	WP_009993137
SP	Q97W02
AlphaFold	Q97W02

BMRB Entry 16869

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	ILE	VAL	LEU	PHE	VAL	ASP	PHE	ASP	TYR
4	PHE	TYR	ALA	GLN	VAL	GLU	GLU	VAL	LEU	ASN
5	PRO	SER	LEU	LYS	GLY	LYS	PRO	VAL	VAL	VAL
6	CYS	VAL	PHE	SER	GLY	ARG	PHE	GLU	ASP	SER
7	GLY	ALA	VAL	ALA	THR	ALA	ASN	TYR	GLU	ALA
8	ARG	LYS	PHE	GLY	VAL	LYS	ALA	GLY	ILE	PRO
9	ILE	VAL	GLU	ALA	LYS	LYS	ILE	LEU	PRO	ASN
10	ALA	VAL	TYR	LEU	PRO	MET	ARG	LYS	GLU	VAL
11	TYR	GLN	GLN	VAL	SER	SER	ARG	ILE	MET	ASN
12	LEU	LEU	ARG	GLU	TYR	SER	GLU	LYS	ILE	GLU
13	ILE	ALA	SER	ILE	ASP	GLU	ALA	TYR	LEU	ASP
14	ILE	SER	ASP	LYS	VAL	ARG	ASP	TYR	ARG	GLU
15	ALA	TYR	ASN	LEU	GLY	LEU	GLU	ILE	LYS	ASN
16	LYS	ILE	LEU	GLU	LYS	GLU	LYS	ILE	THR	VAL
17	THR	VAL	GLY	ILE	SER	LYS	ASN	LYS	VAL	PHE
18	ALA	LYS	ILE	ALA	ALA	ASP	MET	ALA	LYS	PRO
19	ASN	GLY	ILE	LYS	VAL	ILE	ASP	ASP	GLU	GLU
20	VAL	LYS	ARG	LEU	ILE	ARG	GLU	LEU	ASP	ILE
21	ALA	ASP	VAL	PRO	GLY	ILE	GLY	ASN	ILE	THR
22	ALA	GLU	LYS	LEU	LYS	LYS	LEU	GLY	ILE	ASN
23	LYS	LEU	VAL	ASP	THR	LEU	SER	ILE	GLU	PHE
24	ASP	LYS	LEU	LYS	GLY	MET	ILE	GLY	GLU	ALA
25	LYS	ALA	LYS	TYR	LEU	ILE	SER	LEU	ALA	ARG

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	ILE	VAL	LEU	PHE	VAL	ASP	PHE	ASP	TYR
4	PHE	TYR	ALA	GLN	VAL	GLU	GLU	VAL	LEU	ASN
5	PRO	SER	LEU	LYS	GLY	LYS	PRO	VAL	VAL	VAL
6	CYS	VAL	PHE	SER	GLY	ARG	PHE	GLU	ASP	SER
7	GLY	ALA	VAL	ALA	THR	ALA	ASN	TYR	GLU	ALA
8	ARG	LYS	PHE	GLY	VAL	LYS	ALA	GLY	ILE	PRO
9	ILE	VAL	GLU	ALA	LYS	LYS	ILE	LEU	PRO	ASN
10	ALA	VAL	TYR	LEU	PRO	MET	ARG	LYS	GLU	VAL
11	TYR	GLN	GLN	VAL	SER	SER	ARG	ILE	MET	ASN
12	LEU	LEU	ARG	GLU	TYR	SER	GLU	LYS	ILE	GLU
13	ILE	ALA	SER	ILE	ASP	GLU	ALA	TYR	LEU	ASP
14	ILE	SER	ASP	LYS	VAL	ARG	ASP	TYR	ARG	GLU
15	ALA	TYR	ASN	LEU	GLY	LEU	GLU	ILE	LYS	ASN
16	LYS	ILE	LEU	GLU	LYS	GLU	LYS	ILE	THR	VAL
17	THR	VAL	GLY	ILE	SER	LYS	ASN	LYS	VAL	PHE
18	ALA	LYS	ILE	ALA	ALA	ASP	MET	ALA	LYS	PRO
19	ASN	GLY	ILE	LYS	VAL	ILE	ASP	ASP	GLU	GLU
20	VAL	LYS	ARG	LEU	ILE	ARG	GLU	LEU	ASP	ILE
21	ALA	ASP	VAL	PRO	GLY	ILE	GLY	ASN	ILE	THR
22	ALA	GLU	LYS	LEU	LYS	LYS	LEU	GLY	ILE	ASN
23	LYS	LEU	VAL	ASP	THR	LEU	SER	ILE	GLU	PHE
24	ASP	LYS	LEU	LYS	GLY	MET	ILE	GLY	GLU	ALA
25	LYS	ALA	LYS	TYR	LEU	ILE	SER	LEU	ALA	ARG

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	ILE	VAL	LEU	PHE	VAL	ASP	PHE	ASP	TYR
4	PHE	TYR	ALA	GLN	VAL	GLU	GLU	VAL	LEU	ASN
5	PRO	SER	LEU	LYS	GLY	LYS	PRO	VAL	VAL	VAL
6	CYS	VAL	PHE	SER	GLY	ARG	PHE	GLU	ASP	SER
7	GLY	ALA	VAL	ALA	THR	ALA	ASN	TYR	GLU	ALA
8	ARG	LYS	PHE	GLY	VAL	LYS	ALA	GLY	ILE	PRO
9	ILE	VAL	GLU	ALA	LYS	LYS	ILE	LEU	PRO	ASN
10	ALA	VAL	TYR	LEU	PRO	MET	ARG	LYS	GLU	VAL
11	TYR	GLN	GLN	VAL	SER	SER	ARG	ILE	MET	ASN
12	LEU	LEU	ARG	GLU	TYR	SER	GLU	LYS	ILE	GLU
13	ILE	ALA	SER	ILE	ASP	GLU	ALA	TYR	LEU	ASP
14	ILE	SER	ASP	LYS	VAL	ARG	ASP	TYR	ARG	GLU
15	ALA	TYR	ASN	LEU	GLY	LEU	GLU	ILE	LYS	ASN
16	LYS	ILE	LEU	GLU	LYS	GLU	LYS	ILE	THR	VAL
17	THR	VAL	GLY	ILE	SER	LYS	ASN	LYS	VAL	PHE
18	ALA	LYS	ILE	ALA	ALA	ASP	MET	ALA	LYS	PRO
19	ASN	GLY	ILE	LYS	VAL	ILE	ASP	ASP	GLU	GLU
20	VAL	LYS	ARG	LEU	ILE	ARG	GLU	LEU	ASP	ILE
21	ALA	ASP	VAL	PRO	GLY	ILE	GLY	ASN	ILE	THR
22	ALA	GLU	LYS	LEU	LYS	LYS	LEU	GLY	ILE	ASN
23	LYS	LEU	VAL	ASP	THR	LEU	SER	ILE	GLU	PHE
24	ASP	LYS	LEU	LYS	GLY	MET	ILE	GLY	GLU	ALA
25	LYS	ALA	LYS	TYR	LEU	ILE	SER	LEU	ALA	ARG