BMRB Entry 16866

Name: VAMP7(1-118):ISOLATED LONGIN DOMAIN
Published: 2010-04-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16866

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

VAMP7(1-118):ISOLATED LONGIN DOMAIN

Deposition date:

2010-04-14

Original release date:

2010-04-29

Authors:

Vivona, Sandro; Liu, Corey; Strop, Pavel; Rossi, Valeria; Filippini, Francesco; Brunger, Axel

Citation:

Citation: Vivona, Sandro; Liu, Corey; Strop, Pavel; Rossi, Valeria; Filippini, Francesco; Brunger, Axel. "The longin SNARE VAMP7/TI-VAMP adopts a closed conformation." J. Biol. Chem. 285, 17965-17973 (2010).
PubMed: 20378544

Assembly members:

Assembly members:
VAMP7(1-118), polymer, 121 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET47b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VAMP7(1-118): GPGMAILFAVVARGTTILAK HAWCGGNFLEVTEQILAKIP SENNKLTYSHGNYLFHYICQ DRIVYLCITDDDFERSRAFN FLNEIKKRFQTTYGSRAQTA LPYAMNSEFSSVLAAQLKHH S

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	108
1H chemical shifts	108

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	VAMP7(1-118)	1

Entities:

Entity 1, VAMP7(1-118) 121 residues - Formula weight is not available

1

GLY

PRO

GLY

MET

ALA

ILE

LEU

PHE

ALA

VAL

2

VAL

ALA

ARG

GLY

THR

ILE

LEU

ALA

LYS

3

HIS

ALA

TRP

CYS

GLY

ASN

PHE

LEU

GLU

4

VAL

THR

GLU

GLN

ILE

LEU

ALA

LYS

ILE

PRO

5

SER

GLU

ASN

LYS

LEU

THR

TYR

SER

HIS

6

GLY

ASN

TYR

LEU

PHE

HIS

TYR

ILE

CYS

GLN

7

ASP

ARG

ILE

VAL

TYR

LEU

CYS

ILE

THR

ASP

8

ASP

PHE

GLU

ARG

SER

ARG

ALA

PHE

ASN

9

PHE

LEU

ASN

GLU

ILE

LYS

ARG

PHE

GLN

10

THR

TYR

GLY

SER

ARG

ALA

GLN

THR

ALA

11

LEU

PRO

TYR

ALA

MET

ASN

SER

GLU

PHE

SER

12

SER

VAL

LEU

ALA

GLN

LEU

LYS

HIS

13

SER

Samples:

sample_1: VAMP7, [U-100% 15N], 18 uM; D2O 10%; potassium phosphate 20 mM; sodium chloride 100 mM; Dithiothreitol 1 mM; H2O 90%

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	16867
PDB	2DMW 2VX8 4AFI 4B93
DBJ	BAB22386 BAB27667 BAC40712 BAD96514 BAE38126
EMBL	CAA63133 CAA65509 CAB94231 CAB96816 CAC16891
GB	AAF88059 AAH03764 AAH56141 AAI18342 ABK42476
REF	NP_001026292 NP_001069770 NP_001124684 NP_001159676 NP_001172112
SP	P51809 P70280 Q17QI5 Q5RF94 Q5ZL74
TPG	DAA13239
AlphaFold	P51809 P70280 Q17QI5 Q5RF94 Q5ZL74