BMRB Entry 16857
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16857
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Pintar, Alessandro; Guarnaccia, Corrado; Dhir, Somdutta; Pongor, Sandor. "Exon 6 of human JAG1 encodes a conserved structural unit." BMC Struct. Biol. 9, 43-43 (2009).
PubMed: 19586525
Assembly members:
Exon 6 of human Jagged-1, polymer, 44 residues, 5048.796 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Exon 6 of human Jagged-1: RCQYGWQGLYCDKCIPHPGC
VHGICNEPWQCLCETNWGGQ
LCDK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 288 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | JAG1 | 1 |
Entities:
Entity 1, JAG1 44 residues - 5048.796 Da.
Experimentally determined disulfide bonds: 2-11; 14-25; 20-31; 33-42
| 1 | ARG | CYS | GLN | TYR | GLY | TRP | GLN | GLY | LEU | TYR | ||||
| 2 | CYS | ASP | LYS | CYS | ILE | PRO | HIS | PRO | GLY | CYS | ||||
| 3 | VAL | HIS | GLY | ILE | CYS | ASN | GLU | PRO | TRP | GLN | ||||
| 4 | CYS | LEU | CYS | GLU | THR | ASN | TRP | GLY | GLY | GLN | ||||
| 5 | LEU | CYS | ASP | LYS |
Samples:
sample_1: JAG1 0.5 mM; H2O 90%; D2O 10%
sample_2: JAG1 0.5 mM; D2O 100%
sample_conditions_1: pH: 4.5; pressure: 1 atm; temperature: 298 K
sample_conditions_2: pH: 4.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_2 |
Software:
CYANA v2.1, G ntert P. - refinement
NMR spectrometers:
- Bruker Avance 800 MHz