BMRB Entry 16851

Name: The structure of E-protein activation domain 1 bound to the KIX domain of CBP/p300 elucidates leukemia induction by E2A-PBX1
Published: 2012-08-02

Click here to enlarge.

PDB ID: 2kwf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16851
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

The structure of E-protein activation domain 1 bound to the KIX domain of CBP/p300 elucidates leukemia induction by E2A-PBX1

Deposition date:

2010-04-08

Original release date:

2012-08-02

Authors:

Denis, Christopher; Chitayat, Seth; Plevin, Michael; Liu, Shuang; Spencer, Holly; Ikura, Mitsuhiko; LeBrun, David; Smith, Steven

Citation:

Citation: Denis, Christopher; Langelaan, David; Kirlin, Alyssa; Chitayat, Seth; Munro, Kim; Spencer, Holly; LeBrun, David; Smith, Steven. "Functional redundancy between the transcriptional activation domains of E2A is mediated by binding to the KIX domain of CBP/p300." Nucleic Acids Res. ., .-. (2014).
PubMed: 24682819

Assembly members:

Assembly members:
KIX, polymer, 87 residues, 10353.954 Da.
ETAD1, polymer, 19 residues, 2021.198 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet21a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KIX: GVRKGWHEHVTQDLRSHLVH KLVQAIFPTPDPAALKDRRM ENLVAYAKKVEGDMYESANS RDEYYHLLAEKIYKIQKELE EKRRSRL
ETAD1: GSGTDKELSDLLDFSAMFS

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	438
15N chemical shifts	112
1H chemical shifts	656

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KIX	1
2	ETAD1	2

Entities:

Entity 1, KIX 87 residues - 10353.954 Da.

1

GLY

VAL

ARG

LYS

GLY

TRP

HIS

GLU

HIS

VAL

2

THR

GLN

ASP

LEU

ARG

SER

HIS

LEU

VAL

HIS

3

LYS

LEU

VAL

GLN

ALA

ILE

PHE

PRO

THR

PRO

4

ASP

PRO

ALA

LEU

LYS

ASP

ARG

MET

5

GLU

ASN

LEU

VAL

ALA

TYR

ALA

LYS

VAL

6

GLU

GLY

ASP

MET

TYR

GLU

SER

ALA

ASN

SER

7

ARG

ASP

GLU

TYR

HIS

LEU

ALA

GLU

8

LYS

ILE

TYR

LYS

ILE

GLN

LYS

GLU

LEU

GLU

9

GLU

LYS

ARG

SER

ARG

LEU

Entity 2, ETAD1 19 residues - 2021.198 Da.

1

GLY

SER

GLY

THR

ASP

LYS

GLU

LEU

SER

ASP

2

LEU

ASP

PHE

SER

ALA

MET

PHE

SER

Samples:

sample_KIX_ETAD1_1: KIX 1.2 mM; ETAD1, [U-13C; U-15N], 0.6 mM; DSS 1 mM; MES 20 mM; beta-mercaptoethanol 1 mM

sample_KIX_ETAD1_2: KIX, [U-13C; U-15N], 0.6 mM; ETAD1 1.2 mM; DSS 1 mM; MES 20 mM; beta-mercaptoethanol 1 mM

condition_KIX_ETAD1: ionic strength: 0 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_KIX_ETAD1_1	isotropic	condition_KIX_ETAD1
2D 1H-13C HSQC	sample_KIX_ETAD1_1	isotropic	condition_KIX_ETAD1
3D CBCA(CO)NH	sample_KIX_ETAD1_1	isotropic	condition_KIX_ETAD1
3D C(CO)NH	sample_KIX_ETAD1_1	isotropic	condition_KIX_ETAD1
3D HNCO	sample_KIX_ETAD1_1	isotropic	condition_KIX_ETAD1
3D HNCACB	sample_KIX_ETAD1_1	isotropic	condition_KIX_ETAD1
3D HCCH-TOCSY	sample_KIX_ETAD1_1	isotropic	condition_KIX_ETAD1
3D H(CCO)NH	sample_KIX_ETAD1_1	isotropic	condition_KIX_ETAD1
3D 1H-13C NOESY	sample_KIX_ETAD1_1	isotropic	condition_KIX_ETAD1
3D 1H-15N NOESY	sample_KIX_ETAD1_1	isotropic	condition_KIX_ETAD1

Software:

NMRPipe v2.6, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

TOPSPIN, Bruker Biospin - collection

NMRView v5.2.2, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Varian INOVA 600 MHz
Bruker Avance 800 MHz

BMRB	18314 18315 18694 18695 16467
PDB	1KDX 1SB0 2AGH 2KWF 2LQH 2LQI 2LXS 2LXT 2KNH 2KWF
DBJ	BAE06125 BAG65526 BAI45616 BAC26095 BAC38116 BAE21912 BAE21989 BAE25324
GB	AAB28651 AAC08447 AAC51331 AAC51770 AAH72594 AAA42115 AAA58632 AAA60311 AAB32662 AAB62389
PRF	1923401A 2208360B
REF	NP_001020603 NP_001073315 NP_001157494 NP_001247644 NP_004371 NP_001071353 NP_001077431 NP_001077436 NP_001187118 NP_001187247
SP	P45481 Q6JHU9 Q92793 P15884 P51514 Q60722 Q61286 Q99081
TPG	DAA15549 DAA01129 DAA25320
EMBL	CAA46052 CAA62868 CAD89914 CAF92364 CAG00972
AlphaFold	P45481 Q6JHU9 Q92793 P15884 P51514 Q60722 Q61286 Q99081