BMRB Entry 16848

Title:
Solution structure of a novel Ubiquitin-binding domain from Human PLAA (PFUC, Gly76-Pro77 cis isomer).
Deposition date:
2010-04-08
Original release date:
2010-05-10
Authors:
Fu, Qing-Shan
Citation:

Citation: Fu, Qing-Shan; Zhou, Chen-Jie; Gao, Hong-Chang; Jiang, Ya-Jun; Zhou, Zi-Ren; Hong, Jing; Yao, Wen-Ming; Song, Ai-Xin; Lin, Dong-Hai; Hu, Hong-Yu. "Structural basis for ubiquitin recognition by a novel domain from human phospholipase A2-activating protein."  J. Biol. Chem. 284, 19043-19052 (2009).
PubMed: 19423704

Assembly members:

Assembly members:
PFUC-cis, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metzoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-32M

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts221
15N chemical shifts88
1H chemical shifts462

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PFUC1

Entities:

Entity 1, PFUC 80 residues - Formula weight is not available

1   ALAASNGLNGLNTHRSERGLYLYSVALLEU
2   TYRGLUGLYLYSGLUPHEASPTYRVALPHE
3   SERILEASPVALASNGLUGLYGLYPROSER
4   TYRLYSLEUPROTYRASNTHRSERASPASP
5   PROTRPLEUTHRALATYRASNPHELEUGLN
6   LYSASNASPLEUASNPROMETPHELEUASP
7   GLNVALALALYSPHEILEILEASPASNTHR
8   LYSGLYGLNMETLEUGLYLEUGLYASNPRO

Samples:

sample_1: PFUC 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 16818
PDB
DBJ BAA91803 BAA92105 BAD97264 BAE87781 BAG10899
EMBL CAB42881 CAB63739
GB AAD03030 AAD42075 AAH32551 ABW03596 ABW03904
REF NP_001026859 NP_001247477 NP_001270166 XP_002708092 XP_002743032
SP Q9Y263
AlphaFold Q9Y263

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks