BMRB Entry 16840

Name: 1H, 15N and 13C resonance assignment of Darcin, a Major Urinary Protein of the mouse
Published: 2010-08-19

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PDB ID: 2l9c
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16840
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C resonance assignment of Darcin, a Major Urinary Protein of the mouse

Deposition date:

2010-04-06

Original release date:

2010-08-19

Authors:

Phelan, Marie; McLean, Lynn; Simpson, Deborah; Hurst, Jane; Beynon, Robert; Lian, Lu-Yun

Citation:

Citation: Phelan, Marie; McLean, Lynn; Simpson, Deborah; Hurst, Jane; Beynon, Robert; Lian, Lu-Yun. "1H, 15N and 13C resonance assignment of darcin, a mouse major urinary protein." Biomol. NMR Assignments 4, 239-241 (2010).
PubMed: 20703836

Assembly members:

Assembly members:
darcin, polymer, 176 residues, 20533.9058 Da.

Natural source:

Natural source: Common Name: House mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
darcin: MGSSHHHHHHIEGREEASSM ERNFNVEKINGEWYTIMLAT DKREKIEEHGSMRVFVEYIH VLENSLALKFHIIINEECSE IFLVADKTEKAGEYSVTYDG SNTFTILKTDYDNYIMIHLI NKKDGETFQLMELYGREPDL SSDIKEKFAQLSEEHGIVRE NIIDLTNANRCLEARE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	781
15N chemical shifts	186
1H chemical shifts	1202

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	darcin	1

Entities:

Entity 1, darcin 176 residues - 20533.9058 Da.

residues 20 to 181 correspond to the native MUP residues 6 to 19 correspond to the cloning and purification tag

1

MET

GLY

SER

HIS

2

ILE

GLU

GLY

ARG

GLU

ALA

SER

MET

3

GLU

ARG

ASN

PHE

ASN

VAL

GLU

LYS

ILE

ASN

4

GLY

GLU

TRP

TYR

THR

ILE

MET

LEU

ALA

THR

5

ASP

LYS

ARG

GLU

LYS

ILE

GLU

HIS

GLY

6

SER

MET

ARG

VAL

PHE

VAL

GLU

TYR

ILE

HIS

7

VAL

LEU

GLU

ASN

SER

LEU

ALA

LEU

LYS

PHE

8

HIS

ILE

ASN

GLU

CYS

SER

GLU

9

ILE

PHE

LEU

VAL

ALA

ASP

LYS

THR

GLU

LYS

10

ALA

GLY

GLU

TYR

SER

VAL

THR

TYR

ASP

GLY

11

SER

ASN

THR

PHE

THR

ILE

LEU

LYS

THR

ASP

12

TYR

ASP

ASN

TYR

ILE

MET

ILE

HIS

LEU

ILE

13

ASN

LYS

ASP

GLY

GLU

THR

PHE

GLN

LEU

14

MET

GLU

LEU

TYR

GLY

ARG

GLU

PRO

ASP

LEU

15

SER

ASP

ILE

LYS

GLU

LYS

PHE

ALA

GLN

16

LEU

SER

GLU

HIS

GLY

ILE

VAL

ARG

GLU

17

ASN

ILE

ASP

LEU

THR

ASN

ALA

ASN

ARG

18

CYS

LEU

GLU

ALA

ARG

GLU

Samples:

sample_1: darcin, [U-98% 13C; U-98% 15N], 1 mM; potassium phosphate 25 mM; sodium azide 0.2%; H2O 90%; D2O 10%

sample_2: darcin, [U-98% 13C; U-98% 15N], 1 mM; potassium phosphate 25 mM; sodium azide 0.2%; D2O 100%

sample_conditions_1: ionic strength: 25 mM; pH: 6.8; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHANH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D (HB)CB(CGCD)HD	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC (aromatic)	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1

Software:

ANALYSIS v2.1.3, CCPN - chemical shift assignment, data analysis

DANGLE v1.1, CCPN - Secondary structure calculation

AZARA vany, CCPN - Spectrum processing

TOPSPIN v2.1, Bruker biospin - Data collection, Spectrum processing

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz

PDB	2L9C
EMBL	CAP58483 CAQ11567
GB	AAH89613 AAH92096 ACF70718
REF	NP_001012323 XP_006538108
SP	Q5FW60
TPG	DAA06315
AlphaFold	Q5FW60