BMRB Entry 16838

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16838

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Title:
Backbone 1H, 13C, and 15N chemical shift assignments of the extracellular domain of tissue factor
Deposition date:
2010-04-06
Original release date:
2010-06-15
Authors:
Boettcher, John; Clay, Mary; LaHood, Benjamin; Morrissey, James; Rienstra, Chad
Citation:

Citation: Boettcher, John; Clay, Mary; LaHood, Benjamin; Morrissey, James; Rienstra, Chad. "Backbone 1H, 13C and 15N resonance assignments of the extracellular domain of tissue factor."  Biomol. NMR Assignments 4, 183-185 (2010).
PubMed: 20526825

Assembly members:

Assembly members:
soluble_tissue_factor, polymer, 229 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJH677

Entity Sequences (FASTA):

Entity Sequences (FASTA):
soluble_tissue_factor: ATTNTVAAYNLTWKSTNFKT ILEWEPKPVNQVYTVQISTK SGDWKSKCFYTTDTECDLTD EIVKDVKQTYLARVFSYPAG NVESTGSAGEPLYENSPEFT PYLETNLGQPTIQSFEQVGT KVNVTVEDERTLVRRNNTFL SLRDVFGKDLIYTLYYWKSS SSGKKTAKTNTNEFLIDVDK GENYCFSVQAVIPSRTVNRK STDSPVECMGQEKGEFREGG AAGHHHHHH

Data sets:
Data typeCount
13C chemical shifts543
15N chemical shifts204
1H chemical shifts204

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1soluble tissue factor1

Entities:

Entity 1, soluble tissue factor 229 residues - Formula weight is not available

1   ALATHRTHRASNTHRVALALAALATYRASN
2   LEUTHRTRPLYSSERTHRASNPHELYSTHR
3   ILELEUGLUTRPGLUPROLYSPROVALASN
4   GLNVALTYRTHRVALGLNILESERTHRLYS
5   SERGLYASPTRPLYSSERLYSCYSPHETYR
6   THRTHRASPTHRGLUCYSASPLEUTHRASP
7   GLUILEVALLYSASPVALLYSGLNTHRTYR
8   LEUALAARGVALPHESERTYRPROALAGLY
9   ASNVALGLUSERTHRGLYSERALAGLYGLU
10   PROLEUTYRGLUASNSERPROGLUPHETHR
11   PROTYRLEUGLUTHRASNLEUGLYGLNPRO
12   THRILEGLNSERPHEGLUGLNVALGLYTHR
13   LYSVALASNVALTHRVALGLUASPGLUARG
14   THRLEUVALARGARGASNASNTHRPHELEU
15   SERLEUARGASPVALPHEGLYLYSASPLEU
16   ILETYRTHRLEUTYRTYRTRPLYSSERSER
17   SERSERGLYLYSLYSTHRALALYSTHRASN
18   THRASNGLUPHELEUILEASPVALASPLYS
19   GLYGLUASNTYRCYSPHESERVALGLNALA
20   VALILEPROSERARGTHRVALASNARGLYS
21   SERTHRASPSERPROVALGLUCYSMETGLY
22   GLNGLULYSGLYGLUPHEARGGLUGLYGLY
23   ALAALAGLYHISHISHISHISHISHIS

Samples:

sample_1: soluble tissue factor, [U-13C; U-15N; U-2H], 1 mM; sodium phosphate buffer 50 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 90 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian Unity 750 MHz
  • Varian Unity 600 MHz

Related Database Links:

PDB
DBJ BAG35522 BAI46540
EMBL CAG46591
GB AAA36734 AAA61150 AAA61151 AAA61152 AAH11029
REF NP_001171567 NP_001984 XP_001156332 XP_001156450 XP_003808492
SP P13726
AlphaFold P13726

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks