BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16832

Title: Structure of C-terminal domain from mtTyrRS of A. nidulans

Deposition date: 2010-04-02 Original release date: 2012-08-03

Authors: Chari, Nandini

Citation: Hoffman, David; Lambowitz, Alan; Chari, Nandini; Paukstelis, Paul. "Structure of the C-terminal domain of the mtTyrRS from A. nidulans"  .

Assembly members:
mtTyrRS, polymer, 164 residues, 17675.309 Da.

Natural source:   Common Name: Aspergillus nidulellus   Taxonomy ID: 162425   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Emericella nidulans

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11d

Entity Sequences (FASTA):
mtTyrRS: MPRTASPGNPKSSLSGFVNP QSGNPHAPQTNFANMPSARV TLPKSLVYDKTFSKVLWSAG LVASKSEGQRIINNNGAYVG SRPGVKKNEPGGGMPDDLTF TPIKTWNASKTQEFIIDGDL LILKLGKWKMKLVSIVSDEK FKELGLTAPGWDEVVGKGKE EPSP

Data sets:
Data typeCount
13C chemical shifts524
15N chemical shifts137
1H chemical shifts816

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mtTyrRS1

Entities:

Entity 1, mtTyrRS 164 residues - 17675.309 Da.

1   METPROARGTHRALASERPROGLYASNPRO
2   LYSSERSERLEUSERGLYPHEVALASNPRO
3   GLNSERGLYASNPROHISALAPROGLNTHR
4   ASNPHEALAASNMETPROSERALAARGVAL
5   THRLEUPROLYSSERLEUVALTYRASPLYS
6   THRPHESERLYSVALLEUTRPSERALAGLY
7   LEUVALALASERLYSSERGLUGLYGLNARG
8   ILEILEASNASNASNGLYALATYRVALGLY
9   SERARGPROGLYVALLYSLYSASNGLUPRO
10   GLYGLYGLYMETPROASPASPLEUTHRPHE
11   THRPROILELYSTHRTRPASNALASERLYS
12   THRGLNGLUPHEILEILEASPGLYASPLEU
13   LEUILELEULYSLEUGLYLYSTRPLYSMET
14   LYSLEUVALSERILEVALSERASPGLULYS
15   PHELYSGLULEUGLYLEUTHRALAPROGLY
16   TRPASPGLUVALVALGLYLYSGLYLYSGLU
17   GLUPROSERPRO

Samples:

sample_1: entity, [U-100% 15N], 20 mg; entity, [U-100% 13C], 20 mg; sodium chloride 500 mM; TRIS 10 mM; H2O 90%; D2O 10%

sample_2: sodium chloride 500 mM; TRIS 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 500 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H COSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

SPARKY v3.113, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB
GB EAA64829
REF XP_659313
TPE CBF85411

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts