BMRB Entry 16803

Title:
1H, 13C and 15N backbone and side chain resonance assignment of the human RANTES-E66S mutant in a dimeric form
Deposition date:
2010-03-30
Original release date:
2010-04-28
Authors:
Bieri, Andrej; Grzesiek, Stephan
Citation:

Citation: Bieri, Andrej; Grzesiek, Stephan. "1H, 13C and 15N backbone and side chain resonance assignment of the human RANTES-E66S mutant in a dimeric form"  .

Assembly members:

Assembly members:
RANTES-E66S, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEGV2

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts310
15N chemical shifts67
1H chemical shifts473

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1polymer1

Entities:

Entity 1, polymer 68 residues - Formula weight is not available

1   SERPROTYRSERSERASPTHRTHRPROCYS
2   CYSPHEALATYRILEALAARGPROLEUPRO
3   ARGALAHISILELYSGLUTYRPHETYRTHR
4   SERGLYLYSCYSSERASNPROALAVALVAL
5   PHEVALTHRARGLYSASNARGGLNVALCYS
6   ALAASNPROGLULYSLYSTRPVALARGGLU
7   TYRILEASNSERLEUSERMETSER

Samples:

sample_1: D2O 5%; H2O 95%; potassium phosphate 50 mM; sodium azide 0.02%; Rantes-E66S, [U-100% 13C; U-100% 15N], 2 mM

sample_2: D2O 100%; potassium phosphate 50 mM; Rantes-E66S, [U-100% 15N], 0.5 mM

sample_conditions_1: pH: 3.8; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 17453
PDB
DBJ BAG35145 BAG73001
GB AAA36725 AAC03541 AAC63331 AAF73070 AAH08600
REF NP_002976 XP_002827318 XP_003281422 XP_003818023 XP_008009272
SP P13501
AlphaFold P13501

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks