BMRB Entry 16798

Name: 1H, 15N and 13C backbone resonance assignments of domain 3 of the non-structural 5A (NS5A) protein from Hepatitis C Virus (JFH-1)
Published: 2011-05-02

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16798

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C backbone resonance assignments of domain 3 of the non-structural 5A (NS5A) protein from Hepatitis C Virus (JFH-1)

Deposition date:

2010-03-29

Original release date:

2011-05-02

Authors:

Verdegem, Dries; Badillo, Aurelie; Wieruszeski, Jean-Michel; Landrieu, Isabelle; Bartenschlager, Ralf; Penin, Francois; Lippens, Guy; Hanoulle, Xavier

Citation:

Citation: Verdegem, Dries; Badillo, Aurelie; Wieruszeski, Jean-Michel; Landrieu, Isabelle; Leroy, Arnaud; Bartenschlager, Ralf; Penin, Francois; Lippens, Guy; Hanoulle, Xavier. "Domain 3 of NS5A Protein from the Hepatitis C Virus Has Intrinsic {alpha}-Helical Propensity and Is a Substrate of Cyclophilin A." J. Biol. Chem. 286, 20441-20454 (2011).
PubMed: 21489988

Assembly members:

Assembly members:
NS5A-D3, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Hepatitis C Virus Taxonomy ID: 11103 Superkingdom: Virus Kingdom: not available Genus/species: Hepacivirus Hepatitis C Virus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pT7.7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NS5A-D3: MRTVGLSESTISEALQQLAI KTFGQPPSSGDAGSSTGAGA AESGGPTSPGEPAPSETGSA SSMPPLEGEPGDPDLESDQV ELQPPPQGGGVAPGSGSGSW STCSEEDDTTVLQHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	306
15N chemical shifts	97
1H chemical shifts	97

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NS5A-D3	1

Entities:

Entity 1, NS5A-D3 119 residues - Formula weight is not available

1

MET

ARG

THR

VAL

GLY

LEU

SER

GLU

SER

THR

2

ILE

SER

GLU

ALA

LEU

GLN

LEU

ALA

ILE

3

LYS

THR

PHE

GLY

GLN

PRO

SER

GLY

4

ASP

ALA

GLY

SER

THR

GLY

ALA

GLY

ALA

5

ALA

GLU

SER

GLY

PRO

THR

SER

PRO

GLY

6

GLU

PRO

ALA

PRO

SER

GLU

THR

GLY

SER

ALA

7

SER

MET

PRO

LEU

GLU

GLY

GLU

PRO

8

GLY

ASP

PRO

ASP

LEU

GLU

SER

ASP

GLN

VAL

9

GLU

LEU

GLN

PRO

GLN

GLY

10

VAL

ALA

PRO

GLY

SER

GLY

SER

GLY

SER

TRP

11

SER

THR

CYS

SER

GLU

ASP

THR

12

VAL

LEU

GLN

HIS

Samples:

sample_1: HCV (JFH-1) NS5A-D3, [U-95% 13C; U-98% 15N], 500 uM; sodium phosphate 20 mM; sodium chloride 30 mM; sodium azide 0.02%; THP 1 mM; D2O, [U-99.9% 2H], 5%; H2O 95%

sample_conditions_1: ionic strength: 30 mM; pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCANNH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection, processing

In_house_product_plane_algorithm v1.5, Dries Verdegem & Guy Lippens - data analysis

NMR spectrometers:

Bruker Avance 800 MHz

UniProt	Q99IB8
Genbank	AB047639
EuHCVdb	AB047639
BMRB	16799
DBJ	BAB32872 BAD06942 BAF34893
GB	ABX82715 ABY68009 ABY68010 ABY68011 ABY68012
SP	Q99IB8
AlphaFold	Q99IB8 Q99IB8